Bae Ji-Young, Lim Soon Sung, Kim Sun Ju, Choi Jung-Suk, Park Jinseu, Ju Sung Mi, Han Seoung Jun, Kang Il-Jun, Kang Young-Hee
Department of Food and Nutrition and Korean Institute of Nutrition, Hallym University, Chuncheon, Republic of Korea.
Mol Nutr Food Res. 2009 Jun;53(6):726-38. doi: 10.1002/mnfr.200800245.
Fruits of bog blueberry (Vaccinium uliginosum L.) are rich in anthocyanins that contribute pigmentation. Anthocyanins have received much attention as agents with potentials preventing chronic diseases. This study investigated the capacity of anthocyanin-rich extract from bog blueberry (ATH-BBe) to inhibit photoaging in UV-B-irradiated human dermal fibroblasts. BBe anthocyanins were detected as cyanidin-3-glucoside, petunidin-3-glucoside, malvidin-3-glucoside, and delphinidin3-glucoside. ATH-BBe attenuated UV-B-induced toxicity accompanying reactive oxygen species (ROS) production and the resultant DNA damage responsible for activation of p53 and Bad. Preincubation of ATH-BBe markedly suppressed collagen degradation via blunting production of collagenolytic matrix metalloproteinases (MMP). Additionally, ATH-BBe enhanced UV-B-downregulated procollagen expression at transcriptional levels. We next attempted to explore whether ATH-BBe mitigated the MMP-promoted collagen degradation through blocking nuclear factor kappaB (NF-kappaB) activation and MAPK-signaling cascades. UV-B radiation enhanced nuclear translocation of NF-kappaB, which was reversed by treatment with ATH-BBe. The UV-B irradiation rapidly activated apoptosis signal-regulating kinase-1 (ASK-1)-signaling cascades of JNK and p38 mitogen-activated protein kinase (p38 MAPK), whereas ATH-BBe hampered phosphorylation of c-Jun, p53, and signal transducers and activators of transcription-1 (STAT-1) linked to these MAPK signaling pathways. ATH-BBe diminished UV-B augmented-release of inflammatory interleukin (IL)-6 and IL-8. These results demonstrate that ATH-BBe dampens UV-B-triggered collagen destruction and inflammatory responses through modulating NF-kappaB-responsive and MAPK-dependent pathways. Therefore, anthocyanins from edible bog blueberry may be protective against UV-induced skin photoaging.
沼泽蓝莓（Vaccinium uliginosum L.）果实富含花青素，这些花青素赋予了果实颜色。花青素作为具有预防慢性病潜力的物质受到了广泛关注。本研究调查了沼泽蓝莓富含花青素的提取物（ATH-BBe）对紫外线B照射的人皮肤成纤维细胞光老化的抑制能力。检测到沼泽蓝莓花青素为矢车菊素-3-葡萄糖苷、矮牵牛素-3-葡萄糖苷、锦葵色素-3-葡萄糖苷和飞燕草素-3-葡萄糖苷。ATH-BBe减轻了紫外线B诱导的毒性，同时减少了活性氧（ROS）的产生以及由此导致的DNA损伤，而这种DNA损伤会激活p53和Bad。ATH-BBe的预孵育通过抑制胶原酶基质金属蛋白酶（MMP）的产生，显著抑制了胶原蛋白的降解。此外，ATH-BBe在转录水平上增强了紫外线B下调的前胶原表达。接下来，我们试图探究ATH-BBe是否通过阻断核因子κB（NF-κB）激活和丝裂原活化蛋白激酶（MAPK）信号级联反应来减轻MMP促进的胶原蛋白降解。紫外线B辐射增强了NF-κB的核转位，而ATH-BBe处理可使其逆转。紫外线B照射迅速激活了凋亡信号调节激酶-1（ASK-1）-JNK和p38丝裂原活化蛋白激酶（p38 MAPK）的信号级联反应，而ATH-BBe则阻碍了与这些MAPK信号通路相关的c-Jun、p53以及信号转导和转录激活因子-1（STAT-1）的磷酸化。ATH-BBe减少了紫外线B增强释放的炎症性白细胞介素（IL）-6和IL-8。这些结果表明，ATH-BBe通过调节NF-κB反应性和MAPK依赖性途径，减轻了紫外线B引发的胶原蛋白破坏和炎症反应。因此，可食用沼泽蓝莓中的花青素可能对紫外线诱导的皮肤光老化具有保护作用。
