Hernot David C, Boileau Thomas W, Bauer Laura L, Middelbos Ingmar S, Murphy Michael R, Swanson Kelly S, Fahey George C
University of Illinois, Department of Animal Sciences, 1207 West Gregory Drive, Urbana, Illinois 61801, USA.
J Agric Food Chem. 2009 Feb 25;57(4):1354-61. doi: 10.1021/jf802484j.
It is of interest to benefit from the positive intestinal health outcomes of prebiotic consumption but with minimal gas production. This study examined gas production potential, fermentation profile, and microbial modulation properties of several types of oligosaccharides. Substrates studied included short-chain, medium-chain, and long-chain fructooligosaccharides, oligofructose-enriched inulin, galactooligosaccharide, and polydextrose. Each substrate was fermented in vitro using human fecal inoculum, and fermentation characteristics were quantified at 0, 4, 8, and 12 h. Gas and short-chain fatty acid (SCFA) production data showed that short-chain oligosaccharides were more rapidly fermented and produced more SCFA and gas than substrates with greater degrees of polymerization. Lactobacilli increased similarly among substrates. Short-chain oligosaccharides fermentation resulted in the greatest increase in bifidobacteria concentrations. Mixing short- and long-chain oligosaccharides attenuated short-chain oligosaccharide fermentation rate and extent. This study provides new information on the fermentation characteristics of some oligosaccharides used in human nutrition.
从益生元消费对肠道健康的积极影响中获益，同时尽量减少气体产生，这是很有意义的。本研究考察了几种低聚糖的产气潜力、发酵特性和微生物调节特性。所研究的底物包括短链、中链和长链低聚果糖、富含低聚果糖的菊粉、低聚半乳糖和聚葡萄糖。每种底物都使用人粪便接种物进行体外发酵，并在0、4、8和12小时对发酵特性进行定量分析。气体和短链脂肪酸（SCFA）产生的数据表明，与聚合度更高的底物相比，短链低聚糖发酵更快，产生的SCFA和气体更多。各底物中乳酸杆菌的增加情况相似。短链低聚糖发酵导致双歧杆菌浓度增加最多。混合短链和长链低聚糖会减弱短链低聚糖的发酵速率和程度。本研究提供了关于人类营养中使用的一些低聚糖发酵特性的新信息。
