使用iTRAQ标记技术检测人β细胞在胰岛样分化过程中的差异蛋白质组。

Detection of differential proteomes of human beta-cells during islet-like differentiation using iTRAQ labeling.

作者信息

Jin Jonghwa, Park Jungeun, Kim Kyunggon, Kang Yup, Park Sang Gyu, Kim Jae Hyeon, Park Kyong Soo, Jun Heesook, Kim Youngsoo

机构信息

Departments of Biomedical Sciences and Internal Medicine, Genome Research Center for Diabetes and Endocrine Disease, Seoul National University College of Medicine, 28 Yongon-Dong, Seoul 110-799, Korea.

出版信息

J Proteome Res. 2009 Mar;8(3):1393-403. doi: 10.1021/pr800765t.

DOI:10.1021/pr800765t

PMID:19199707

Abstract

A human beta-cell line, RNAKT-15, was recently established from human pancreatic islets, whereby its differentiation into islet-like beta-cells (islet-like RNAKT-15) increased its expression of insulin 2-fold compared with RNAKT-15 cells. To characterize the differentiation of RNAKT-15 cells into islet-like RNAKT-15, microarray and quantitative proteomics were performed. Our analysis of differential proteomic and mRNA expression has resulted in a greater understanding of the molecular functions that are involved in beta-cell differentiation and insulin synthesis and release.

摘要

一种人β细胞系RNAKT-15最近从人胰岛中建立，与RNAKT-15细胞相比，其向胰岛样β细胞（胰岛样RNAKT-15）的分化使胰岛素表达增加了2倍。为了表征RNAKT-15细胞向胰岛样RNAKT-15的分化，进行了微阵列和定量蛋白质组学分析。我们对差异蛋白质组和mRNA表达的分析使我们对β细胞分化以及胰岛素合成和释放所涉及的分子功能有了更深入的了解。