用烷基化试剂甲磺酸乙酯诱导原噬菌体

Prophage induction by alkylating ethyl methylaminosulfonate.

作者信息

Burger K, Süssmuth R

出版信息

Z Naturforsch C Biosci. 1977 Jan-Feb;32(1-2):140-2.

PMID:192005

Abstract

Treatment of the lysogenic strain M. lysodeikticus 53-40 (N5) with ethyl methylaminosulfonate results in a slight increase in infective center concentration of N5 phages which is only detectable once disturbing cells had been eliminated by lysozyme. After conversion of ethyl methylaminosulfonate with radioactive labelled guanosine at 37 degrees C, N7-ethyl-guanine could be ascertained. Therefore the biological activity of the drug may be due to an ethylation of DNA.

摘要

用甲基氨基磺酸乙酯处理溶源性菌株溶壁微球菌53 - 40（N5），会导致N5噬菌体的感染中心浓度略有增加，这种增加只有在通过溶菌酶消除干扰细胞后才能检测到。在37摄氏度下用放射性标记的鸟苷转化甲基氨基磺酸乙酯后，可以确定N7 - 乙基鸟嘌呤。因此，该药物的生物活性可能是由于DNA的乙基化作用。