Acosta-Madrid I I, Castañeda-Hernández G, Cilia-López V G, Cariño-Cortés R, Pérez-Hernández N, Fernández-Martínez E, Ortiz M I
Centro de Investigaciones Biológicas del Instituto de Ciencias Básicas e Ingenierías, Universidad Autónoma del Estado de Hidalgo, Pachuca, Hidalgo, Mexico.
Phytomedicine. 2009 Apr;16(4):336-41. doi: 10.1016/j.phymed.2008.12.014. Epub 2009 Feb 5.
Heliopsis longipes is an herbaceous plant found in Mexico, used traditionally for its analgesic and anesthetic activities. Plant extracts in combined use with synthetic drugs may represent a therapeutic advantage for the clinical treatment of pain, allowing the use of lower doses, and limiting side-effects. Therefore, the main objective of this study was to determine the possible pharmacological interaction between Heliopsis longipes ethanolic extract (HLEE) and diclofenac in the Hargreaves model of thermal hyperalgesia in the mouse. HLEE, diclofenac or fixed-dose ratio HLEE-diclofenac combinations were administered systemically to mice and the antihyperalgesic effect was evaluated using the thermal hyperalgesia test. All treatments produced a dose-dependent antihyperalgesic effect. ED(30) values were estimated for all the treatments and an isobologram was constructed. The derived theoretical ED(30) value for the HLEE-diclofenac combination was 54.4+/-9.4 mg/kg body wt, significantly higher than the actually observed experimental ED(30) value, 8.6+/-4.0 mg/kg body wt. This result corresponds to synergistic interaction between HLEE and diclofenac in the Hargreaves model of thermal hyperalgesia. Data suggest that low doses of the HLEE-diclofenac combination can interact synergistically at the systemic level and that this association may therefore represent a therapeutic advantage for the clinical treatment of inflammatory pain.
长柄堆心菊是一种在墨西哥发现的草本植物，传统上因其镇痛和麻醉作用而被使用。植物提取物与合成药物联合使用可能对疼痛的临床治疗具有治疗优势，允许使用较低剂量，并限制副作用。因此，本研究的主要目的是在小鼠热痛觉过敏的哈格里夫斯模型中确定长柄堆心菊乙醇提取物（HLEE）与双氯芬酸之间可能的药理相互作用。将HLEE、双氯芬酸或固定剂量比例的HLEE-双氯芬酸组合全身给药于小鼠，并使用热痛觉过敏试验评估其抗痛觉过敏作用。所有治疗均产生剂量依赖性抗痛觉过敏作用。估计了所有治疗的ED(30)值并构建了等效应线图。HLEE-双氯芬酸组合的推导理论ED(30)值为54.4±9.4mg/kg体重，显著高于实际观察到的实验ED(30)值8.6±4.0mg/kg体重。该结果对应于在热痛觉过敏的哈格里夫斯模型中HLEE与双氯芬酸之间的协同相互作用。数据表明，低剂量的HLEE-双氯芬酸组合在全身水平上可协同相互作用，因此这种联合可能对炎性疼痛的临床治疗具有治疗优势。
