Mendes R K, Carvalhal R F, Stach-Machado D R, Kubota L T
Institute of Chemistry, State University of Campinas, 13083-970, PO Box 6154, 13083-970 Campinas, SP, Brazil.
Biosens Bioelectron. 2009 Apr 15;24(8):2483-7. doi: 10.1016/j.bios.2008.12.033. Epub 2008 Dec 30.
Soybean rust (Asian rust) is a disease that occurs in soy cultures, negatively affecting pod formation and final grain weight and reducing value and product quality. Early identification of fungus in the plants prevents severe farming losses and spread to neighboring cultures. In this paper, a fast response sensor was developed based on surface plasmon resonance to detect Asian rust in soybean leaf extract at early stages of the disease. The antibody anti-Phakopsora pachyrhizi (pathogen) was covalently immobilized on a gold substrate via a self-assembled monolayer (SAM) of thiols using cysteamine-coupling chemistry. This immunosensor presented a linear response range for the antigen from 3.5 to 28.0 microg mL(-1) (r(2)=0.996). The effects of the antibody amount and the surface blocking to minimize non-specific adsorption on immunosensor response were evaluated. These studies provide new perspectives on using SPR technology for the development of a highly sensitive sensor for agricultural applications.
大豆锈病（亚洲锈病）是一种发生在大豆作物上的病害，会对豆荚形成和最终籽粒重量产生负面影响，降低价值和产品质量。早期识别植物中的真菌可防止严重的农业损失并防止病害传播到邻近作物。本文基于表面等离子体共振开发了一种快速响应传感器，用于在病害早期检测大豆叶片提取物中的亚洲锈病。通过使用半胱胺偶联化学的硫醇自组装单分子层（SAM），将抗大豆锈病菌（病原体）抗体共价固定在金基底上。该免疫传感器对抗抗原的线性响应范围为3.5至28.0微克/毫升（r² = 0.996）。评估了抗体量和表面封闭对最小化非特异性吸附对免疫传感器响应的影响。这些研究为利用表面等离子体共振技术开发用于农业应用的高灵敏度传感器提供了新的视角。
