Shoji Yoko, Farrance Christine E, Bi Hong, Shamloul Moneim, Green Brian, Manceva Slobodanka, Rhee Amy, Ugulava Natalia, Roy Gourgopal, Musiychuk Konstantin, Chichester Jessica A, Mett Vadim, Yusibov Vidadi
Fraunhofer USA Center for Molecular Biotechnology, 9 Innovation Way, Suite 200, Newark, DE 19711, USA.
Vaccine. 2009 May 26;27(25-26):3467-70. doi: 10.1016/j.vaccine.2009.01.051. Epub 2009 Feb 5.
Highly pathogenic avian influenza (HPAI) viruses of the H5N1 subtype have been identified as a potential pandemic threat by the World Health Organization (WHO). Since 1997, these viruses have been spreading from Asia to Europe and Africa with increasing genetic and antigenic diversities. Vaccination is the preferred strategy for the prevention and control of influenza infections and the availability of a system for the rapid engineering and production of vaccines is required in the event of an influenza pandemic. In this study, we engineered and produced recombinant hemagglutinin (HA) from A/Bar-headed Goose/Qinghai/1A/05 (clade 2.2) and A/Anhui/1/2005 (clade 2.3) in Nicotiana benthamiana plants. Immunization of mice with these plant-derived HA antigens elicited serum hemagglutination inhibition (HI) and virus neutralization (VN) antibodies. These results suggest the utility of our plant-expression system for recombinant influenza vaccine production.
H5N1亚型高致病性禽流感（HPAI）病毒已被世界卫生组织（WHO）确定为一种潜在的大流行威胁。自1997年以来，这些病毒已从亚洲传播到欧洲和非洲，其遗传和抗原多样性不断增加。疫苗接种是预防和控制流感感染的首选策略，在流感大流行时需要有一个快速设计和生产疫苗的系统。在本研究中，我们在本氏烟草植物中设计并生产了来自A/斑头雁/青海/1A/05（2.2分支）和A/安徽/1/2005（2.3分支）的重组血凝素（HA）。用这些植物源HA抗原免疫小鼠可诱导血清血凝抑制（HI）和病毒中和（VN）抗体。这些结果表明我们的植物表达系统在重组流感疫苗生产中的实用性。
