Lacunza Ezequiel, Abba Martín C, Segal-Eiras Amada, Croce María V
Center for Basic and Applied Immunological Research, Faculty of Medical Sciences, National University of La Plata, Calle 60 y 120, 1900 La Plata, Argentina.
Vet Immunol Immunopathol. 2009 Jul 15;130(1-2):17-24. doi: 10.1016/j.vetimm.2009.01.001. Epub 2009 Jan 6.
Many pathogens require direct binding to, or penetration of, mucosal cells to cause pathology. Cell surface mucins are critical components of mucosal defense. Mucin 1, named MUC1 in humans and Muc1 in non-human species, is a transmembrane glycoprotein expressed in apical mammalian epithelial tissues. The aim of this study was to determine the Muc1 profile expression in healthy cat epithelial tissues. An extensive analysis of Muc1 expression was performed by immunohistochemistry (IHC), Western blot (WB) and RT-PCR. By IHC, the presence of Muc1 protein was observed in the epithelial cells of the esophagus, stomach, trachea, lung, small and large intestine, liver, pancreas, salivary glands, lactating mammary glands and bladder. The predominantly linear patterns of reaction as well as the ubiquitous expression of feline Muc1 were consistent with normal human tissues. By WB, a band of 35kDa, corresponding to that predicted for the Muc1 cytoplasmic tail, was detected. The RT-PCR analysis showed a fragment of 115bp, consistent with that found in MCF7 and T47D human cell lines. The results showed that the widest distribution of feline Muc1 expression is in the mucosal tissues most at risk of infections such as the gastrointestinal tract, respiratory tract and lactating mammary gland. This study provides a normal model of cat Muc1 pattern expression as a starting point to evaluate and compare the expression of this epithelial mucin in pathological feline tissues. We believe that the CT33 antibody and the universal primers designed could be valuable tools for veterinary pathologists involved in the diagnostic interpretation of alterations in Muc1 expression of infected tissues in cats.
许多病原体需要直接结合或穿透黏膜细胞才能引发病变。细胞表面黏蛋白是黏膜防御的关键组成部分。黏蛋白1，在人类中称为MUC1，在非人类物种中称为Muc1，是一种跨膜糖蛋白，在哺乳动物顶端上皮组织中表达。本研究的目的是确定健康猫上皮组织中Muc1的表达谱。通过免疫组织化学（IHC）、蛋白质免疫印迹（WB）和逆转录聚合酶链反应（RT-PCR）对Muc1的表达进行了广泛分析。通过IHC，在食管、胃、气管、肺、小肠和大肠、肝脏、胰腺、唾液腺、泌乳乳腺和膀胱的上皮细胞中观察到Muc1蛋白的存在。猫Muc1主要呈线性反应模式且广泛表达，这与正常人体组织一致。通过WB，检测到一条35kDa的条带，与预测的Muc1细胞质尾相对应。RT-PCR分析显示有一个115bp的片段，与在MCF7和T47D人细胞系中发现的片段一致。结果表明，猫Muc1表达分布最广泛的是在感染风险最高的黏膜组织，如胃肠道、呼吸道和泌乳乳腺。本研究提供了猫Muc1模式表达的正常模型，作为评估和比较这种上皮黏蛋白在猫病理组织中表达的起点。我们认为，所设计的CT33抗体和通用引物可能是兽医病理学家在诊断解释猫感染组织中Muc1表达改变时的有价值工具。
