多种哺乳动物物种的上皮组织中保守黏蛋白结构域的表达。
Expression of conserved mucin domains by epithelial tissues in various mammalian species.
作者信息
Lacunza Ezequiel, Bara Jacques, Segal-Eiras Amada, Croce María V
机构信息
Centre of Basic and Applied Immunological Research, Faculty of Medical Sciences, National University of La Plata, Calle 60 y 120, 1900 La Plata, Argentina.
出版信息
Res Vet Sci. 2009 Feb;86(1):68-77. doi: 10.1016/j.rvsc.2008.05.011. Epub 2008 Jun 25.
UNLABELLED
Mucins are related to infectious and non-infectious diseases in Veterinary and Human Medicine. MUC1 mucin is a transmembrane glycoprotein expressed on the apical surface of human epithelia while MUC5AC is the predominant secreted mucin expressed in human gastric epithelium and goblet cells of lung and eyes. MUC5AC C-terminus cysteine rich regions and the cytoplasmic tail of MUC1 domains are conserved among several mammalian species.
OBJECTIVE
to compare the expression of MUC1 and MUC5AC mucins in mammalian epithelia. CT33 anti-MUC1 cytoplasmic tail (MUC1CT) polyclonal antibody and 45M1 anti-MUC5AC monoclonal antibody were employed. By immunohistochemistry, MUC1CT was expressed in most tissues while MUC5AC was restricted to gastric surface epithelium and goblet cells from trachea and lung. By western blot, MUC1CT showed a band at approximately 35 kDa in most tissues; MUC5AC revealed bands at >180 kDa in stomach and lung secretions from rat, cat, pig and cow. When rat MUC5AC was immunoprecipitated, a band at about 180 kDa was obtained.
未标记
黏蛋白与兽医和人类医学中的感染性和非感染性疾病相关。MUC1黏蛋白是一种跨膜糖蛋白，表达于人类上皮细胞的顶端表面，而MUC5AC是在人类胃上皮以及肺和眼的杯状细胞中表达的主要分泌型黏蛋白。MUC5AC的C末端富含半胱氨酸区域以及MUC1结构域的细胞质尾部在几种哺乳动物物种中是保守的。
目的
比较MUC1和MUC5AC黏蛋白在哺乳动物上皮中的表达。使用CT33抗MUC1细胞质尾部（MUC1CT）多克隆抗体和45M1抗MUC5AC单克隆抗体。通过免疫组织化学，MUC1CT在大多数组织中表达，而MUC5AC局限于胃表面上皮以及气管和肺的杯状细胞。通过蛋白质印迹法，MUC1CT在大多数组织中显示出一条约35 kDa的条带；MUC5AC在大鼠、猫、猪和牛的胃和肺分泌物中显示出大于180 kDa的条带。当对大鼠MUC5AC进行免疫沉淀时，获得了一条约180 kDa的条带。
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