Jadhav Kisan B, Rajini Padmanabhan Sharda
Food Protectants and Infestation Control Department, Central Food Technological Research Institute (CSIR lab), Mysore, India.
J Biochem Mol Toxicol. 2009 Jan-Feb;23(1):9-17. doi: 10.1002/jbt.20258.
The primary objective of this study was to examine a possible correlation among the three endpoints of toxicity, namely, stress gene expression (hsp16), feeding, and acetylcholinesterase (AChE) activity in transgenic C. elegans (hsp16-lacZ) exposed to sublethal concentrations of dichlorvos, an organophosphorus insecticide. Worms exposed to dichlorvos (at 5, 40, and 80 microM) exhibited a concentration-dependent inhibition in feeding with total cessation in feeding occurring beyond 4 h of exposure. Concomitantly, marked and dose-dependent inhibition (69%-90%) of AChE was also evident after 4 h of exposure. Induction of heat shock protein (Hsp) was evident after 4 h of exposure (as seen from quantitative analysis), although maximum expression of Hsp was evident only after 24 h of exposure (as evident from qualitative analysis). Interestingly, the Hsp induction was restricted only to the pharyngeal region. Significant correlation was discernible between the three evaluated end points suggesting their possible interrelated role in the physiological dysfunctions evoked by sublethal concentrations of dichlorvos.
本研究的主要目的是检测暴露于亚致死浓度敌敌畏（一种有机磷杀虫剂）的转基因秀丽隐杆线虫（hsp16-lacZ）的三个毒性终点之间的可能相关性，这三个终点分别是应激基因表达（hsp16）、摄食以及乙酰胆碱酯酶（AChE）活性。暴露于敌敌畏（5、40和80微摩尔）的线虫在摄食方面呈现出浓度依赖性抑制，暴露超过4小时后摄食完全停止。与此同时，暴露4小时后，AChE也出现了显著的剂量依赖性抑制（69%-90%）。暴露4小时后热休克蛋白（Hsp）诱导明显（从定量分析可见），尽管Hsp的最大表达仅在暴露24小时后明显（从定性分析可见）。有趣的是，Hsp诱导仅限于咽部区域。在三个评估终点之间可看出显著相关性，表明它们在亚致死浓度敌敌畏引起的生理功能障碍中可能具有相互关联的作用。
