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用冷冻解冻后的马鹿精液对马鹿(赤鹿)进行人工授精。

Artificial insemination of red deer (Cervus elaphus) with frozen-thawed wapiti semen.

作者信息

Haigh J C, Bowen G

机构信息

Department of Herd Medicine and Theriogenology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.

出版信息

J Reprod Fertil. 1991 Sep;93(1):119-23. doi: 10.1530/jrf.0.0930119.

DOI:10.1530/jrf.0.0930119
PMID:1920280
Abstract

Semen collected from wapiti (Cervus elaphus) in Canada in 1983 was frozen in two extenders. In 1988, the semen was used to inseminate 200 red deer hinds on 2 farms in New Zealand. Oestrus was synchronized in the hinds with progesterone-impregnated intravaginal devices (CIDR); 200 iu pregnant mares' serum gonadotrophin was given to each hind on Day 11. The CIDRs were removed on Day 12 at 20/h, as the numbers of the hinds were recorded. On Day 14, 54-56 h after CIDR removal, the hinds were brought into the yards in the same batches and laparoscopically inseminated. Semen from three sires was used. The overall conception rate was 51%. Gestation length ranged from 239 to 247 days. One hind was lost at calving, 3 calves had to be hand raised and there were 2 neonatal calf deaths.

摘要

1983年从加拿大马鹿(Cervus elaphus)采集的精液用两种稀释液冷冻保存。1988年,这些精液被用于给新西兰两个农场的200头母鹿输精。用含孕酮的阴道内装置(CIDR)使母鹿发情同期化;在第11天给每头母鹿注射200国际单位的孕马血清促性腺激素。在第12天以每小时20头的速度取出CIDR,并记录母鹿数量。在取出CIDR后的54 - 56小时,即第14天,将母鹿按批次赶入围场,通过腹腔镜进行输精。使用了来自三头种公鹿的精液。总体受胎率为51%。妊娠期为239至247天。产犊时有一头母鹿死亡,3头犊牛需要人工饲养,还有2头新生犊牛死亡。

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