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马鹿(Cervus elaphus)和麋鹿(Elaphurus davidianus)的超数排卵,以及用麋鹿精液人工授精后的受精率。

Superovulation in red deer (Cervus elaphus) and Père David's deer (Elaphurus davidianus), and fertilization rates following artificial insemination with Père David's deer semen.

作者信息

Argo C M, Jabbour H N, Goddard P J, Webb R, Loudon A S

机构信息

Institute of Zoology, Zoological Society of London, UK.

出版信息

J Reprod Fertil. 1994 Mar;100(2):629-36. doi: 10.1530/jrf.0.1000629.

DOI:10.1530/jrf.0.1000629
PMID:8021886
Abstract

Two comparative studies were undertaken using adult, female red and Père David's deer to examine the ovulatory response of these animals to a superovulation regimen and fertilization rates following inter- and intraspecific laparoscopic insemination. In Expt 1 six Père David's deer and 12 red deer hinds were treated during the breeding season with an intravaginal progesterone-impregnated controlled internal drug release device (CIDR) for 14 days, with 200 iu pregnant mares' serum gonadotrophin (PMSG) administered 72 h before the device was withdrawn and eight injections of ovine FSH given at 12 h intervals starting at the time of PMSG administration. Oestrous behaviour began one day after CIDR device withdrawal (Père David's deer: 24.00 +/- 2.32 h; red deer: 24.60 +/- 2.23 h). The duration of oestrus was greater in Père David's deer than in red deer (17.50 +/- 1.43 h and 8.25 +/- 3.25 h, respectively, P < 0.001). The peak LH surge of Père David's deer was 68.65 +/- 4.74 ng ml-1 occurring 29.00 +/- 2.41 h after removal of the CIDR devices. In comparison, the peak LH surge in red deer was 17.09 +/- 3.64 ng ml-1 (P < 0.001), occurring 24.00 +/- 0.00 h after CIDR device withdrawal. Pre-surge concentrations of LH were also greater (P < 0.001) in Père David's deer (1.37 +/- 0.11 ng ml-1) than in red deer hinds (0.41 +/- 0.02 ng ml-1).(ABSTRACT TRUNCATED AT 250 WORDS)

摘要

进行了两项比较研究,使用成年雌性马鹿和麋鹿来检查这些动物对超数排卵方案的排卵反应以及种间和种内腹腔镜授精后的受精率。在实验1中,在繁殖季节,六只麋鹿和12只马鹿母鹿用阴道内孕酮浸渍的控制内部药物释放装置(CIDR)处理14天,在取出该装置前72小时给予200国际单位孕马血清促性腺激素(PMSG),并从给予PMSG时开始每隔12小时注射八次绵羊促卵泡素。CIDR装置取出后一天开始出现发情行为(麋鹿:24.00±2.32小时;马鹿:24.60±2.23小时)。麋鹿的发情持续时间比马鹿长(分别为17.50±1.43小时和8.25±3.25小时,P<0.001)。麋鹿的促黄体生成素(LH)峰值为68.65±4.74纳克/毫升,在取出CIDR装置后29.00±2.41小时出现。相比之下,马鹿的LH峰值为17.09±3.64纳克/毫升(P<0.001),在取出CIDR装置后24.00±0.00小时出现。麋鹿LH激增前的浓度(1.37±0.11纳克/毫升)也高于马鹿母鹿(0.41±0.02纳克/毫升)(P<0.001)。(摘要截断于250字)

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