Wang Liping, Zheng Binghui
River and Coastal Environment Research Center, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
J Environ Sci (China). 2008;20(11):1363-72. doi: 10.1016/s1001-0742(08)62234-2.
To investigate the effects of polycyclic aromatic hydrocarbons (PAHs) and metals on the population reproduction, antioxidative defense system and cell ultrastructure of the marine diatom, fluoranthene and Cu2+ were selected to test their toxicity to Phaeodactylum tricorntum, in the laboratory. The results indicated that both fluoranthene and Cu2+ inhibited population reproduction of P. tricorntum. When the algal cells were exposed to fluoranthene or Cu2+ for 72 h, ultrastructure damage in the cells was observed under a Transmission Electron Microscope (TEM). The chloroplast was a sensitive organelle and the membrane system was very sensitive to the toxicity of fluoranthene or Cu2+. The normal metabolic process might be affected due to cell ultrastructural impairment. However, it needed further investigation to discern whether the ultrastructural damage was responsible for the inhibition of population reproduction. Malondialdehyde (MDA) content indicating oxidative stress was a sensitive index to both fluoranthene and Cu2+ toxicity. Superoxide dismutase (SOD) activity was also a sensitive index to Cu2+ and could be considered as a reference factor in a Cu2+ pollution event, but not in a fluoranthene pollution event.
为研究多环芳烃（PAHs）和金属对海洋硅藻种群繁殖、抗氧化防御系统及细胞超微结构的影响，在实验室中选取荧蒽和Cu2+测试它们对三角褐指藻的毒性。结果表明，荧蒽和Cu2+均抑制三角褐指藻的种群繁殖。当藻类细胞暴露于荧蒽或Cu2+ 72小时后，在透射电子显微镜（TEM）下观察到细胞超微结构损伤。叶绿体是一个敏感的细胞器，膜系统对荧蒽或Cu2+的毒性非常敏感。细胞超微结构损伤可能会影响正常的代谢过程。然而，超微结构损伤是否是种群繁殖受抑制的原因还需要进一步研究。表明氧化应激的丙二醛（MDA）含量是对荧蒽和Cu2+毒性均敏感的指标。超氧化物歧化酶（SOD）活性也是对Cu2+敏感的指标，可作为Cu2+污染事件中的参考因素，但在荧蒽污染事件中则不然。
