Shen Huan-si, Chen Chao-wei, Li Yu-mei, Chen Yong-qiang
Department of Orthopaedics and Traumatology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai 200071, China.
Zhongguo Gu Shang. 2009 Jan;22(1):14-7.
To observe the effects of Nourishing yin clearing heat ([Chinese characters: see text]) Chinese herbal medicine on vascular endothelial growth factor (VEGF) in growth retardation induced by Decamethasone and observe its mechanisms.
Thirty one-month-old New Zealand white rabbits were randomly divided into normal group, dexamethasone-treated group and Nourishing yin clearing heat ([Chinese characters: see text]) Chinese herbal medicine-treated group. The rabbits in dexamethasone group and Nourishing yin clearing heat ([Chinese characters: see text]) Chinese herbal medicine-treated group received dexamethasone (5 mg/kg x d). The rabbits were sacrificed at the 6th and 12th week after administration, and then rabbit tibia articular was removed. (1) Using TUNEL stain to detect apoptotic index. (2) Using immunohistochemical stain to detect the positive index of the expression of vascular endothelial growth factor (VEGF) in the epiphyseal cartilage of growth. (3) Using fluorescent quantitative PCR to detect the expression intensity of VEGF mRNA in each group.
At the 6th and 12th week after administration, there were significant difference in apoptotic index and cell proliferation index between dexamethasone group and normal group (P<0.01, dexamethasone group more than normal group). Immunohistochemical stain and fluorescent quantitative PCR indicated that the expression of VEGF and VEGF mRNA in dexamethasone group was significantly decreased as compared with that in normal group (P<0.01), and also obviously lower than Chinese herbal medicine-treated group (P<0.01).
VEGF has 2. an important role during the growth retardation induced by Dexamethasone. Nourishing yin clearing heat ([Chinese characters: see text]) Chinese herbal medicine can reduce the growth retardation induced by Dexamethasone through increasing the VEGF expression in growth plate chondrocytes and then increase angiogenesis.
观察滋阴清热中药对地塞米松致生长迟缓模型血管内皮生长因子（VEGF）的影响并探讨其机制。
将30只1月龄新西兰白兔随机分为正常组、地塞米松组和滋阴清热中药组。地塞米松组和滋阴清热中药组给予地塞米松（5mg/kg·d）。给药后第6、12周处死动物，取兔胫骨近端关节。（1）采用TUNEL染色检测凋亡指数。（2）采用免疫组化染色检测生长板软骨血管内皮生长因子（VEGF）表达阳性指数。（3）采用荧光定量PCR检测各组VEGF mRNA表达强度。
给药后第6、12周，地塞米松组凋亡指数和细胞增殖指数与正常组比较差异有统计学意义（P<0.01，地塞米松组高于正常组）。免疫组化染色及荧光定量PCR结果显示，地塞米松组VEGF及VEGF mRNA表达较正常组显著降低（P<0.01），且明显低于中药组（P<0.01）。
VEGF在糖皮质激素致生长迟缓过程中起重要作用。滋阴清热中药可通过上调生长板软骨细胞VEGF表达，增加血管生成，减轻地塞米松致生长迟缓作用。
