Zhao Wei, Wen Haixia, Zheng Huili, Sun Shixiao, Ni Jiang
Department of Physiology, Harbin Medical University, Harbin 150086, China.
Zhongguo Zhong Yao Za Zhi. 2009 Nov;34(22):2932-6.
To investigate the effect of one kind of regulating-qi and Nourishing-yin Chinese herbs Zuoguiyin on the expression of rats ovarian vascular endothelial growth factor (VEGF) and secreted protein acidic rich in cysteine (SPARC) during the period of peri-menopause.
The animal models of perimenopause rats were established by natural aging. Perimenopause rats were treated by intragastric administration (ig) with low (13.78 g x kg(-1)), middle (20.67 g x kg(-1)) and high (31 g x kg(-1)) dose of Zuoguiyin for 8 weeks. Expression of VEGF and SPARC mRNA were detected by RT-PCR. Immunohistochemistry was used to evaluate expression levels of VEGF protein.
Compared with that in the control group, ovarian expression levels of VEGF mRNA and its protein in rats during peri-menopause increased significantly (P < 0.01). Middle and high dose of Zuoguiyin could both down-regulate the expression of VEGF in ovaries of praesenilis rats, and the difference has statistical significance (P < 0.01). Expression levels of SPARC mRNA in rat ovaries during peri-menopausal period decreased obviously compared to that in the control group (P < 0.01). Middle and high dose of Zuoguiyin could greatly promote ovarian SPARC mRNA expression of praesenilis rats (P < 0.01).
The abnormal changes of VEGF and SPARC may play an important role in the aging process of ovary. Zuoguiyin, one kind of regulating-qi and Nourishing-yin Chinese herbs, can improve ovarian vascular formation through down-regulating the expression of VEGF meanwhile up-regulating the expression of SPARC. And it may delay aging by this way.
探讨一种理气滋阴中药左归丸对围绝经期大鼠卵巢血管内皮生长因子（VEGF）及富含半胱氨酸的酸性分泌蛋白（SPARC）表达的影响。
采用自然衰老法建立围绝经期大鼠动物模型。将围绝经期大鼠分别用低剂量（13.78 g·kg⁻¹）、中剂量（20.67 g·kg⁻¹）和高剂量（31 g·kg⁻¹）的左归丸灌胃给药8周。采用逆转录聚合酶链反应（RT-PCR）检测VEGF和SPARC mRNA的表达。用免疫组织化学法评估VEGF蛋白的表达水平。
与对照组相比，围绝经期大鼠卵巢VEGF mRNA及其蛋白表达水平显著升高（P<0.01）。中、高剂量左归丸均可下调早衰大鼠卵巢VEGF的表达，差异有统计学意义（P<0.01）。围绝经期大鼠卵巢SPARC mRNA表达水平与对照组相比明显降低（P<0.01）。中、高剂量左归丸可显著促进早衰大鼠卵巢SPARC mRNA表达（P<0.01）。
VEGF和SPARC的异常变化可能在卵巢衰老过程中起重要作用。理气滋阴中药左归丸可通过下调VEGF表达同时上调SPARC表达来改善卵巢血管形成，可能以此延缓衰老。
