Zhang Jing, Jin Yu, Dong Jun, Xiao Yuansheng, Feng Jiatao, Xue Xingya, Zhang Xiuli, Liang Xinmiao
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Talanta. 2009 Apr 30;78(2):513-22. doi: 10.1016/j.talanta.2008.12.002. Epub 2008 Dec 6.
Ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS) is an effective technique for analysis of complex samples with offering rapid, efficient separation in combination with accurate mass measurement and tandem mass spectrometry (MS/MS). This paper exploits this technique to identify the alkaloids in corydalis yanhusuo, an important antalgic Traditional Chinese Medicine (TCM). The mass spectral fragmentation behavior of one tertiary alkaloid and two quaternary alkaloids was studied in detail. Low-abundance product ions of tertiary and quaternary alkaloids were investigated and compared between each other. Sixteen alkaloids were screened out by using a systematic screening method developed in our laboratory; structures of eight therein were identified by characteristic UV absorption spectrum and positive ion mode of Q-TOF-MS/MS; and two of them were discovered for the first time in corydalis yanhusuo to our knowledge. This research demonstrates the potential of UPLC-Q-TOF-MS in structural characterization and identification of components in traditional Chinese herbal medicines.
超高效液相色谱-四极杆-飞行时间质谱联用仪（UPLC-Q-TOF-MS）是一种用于分析复杂样品的有效技术，它能够快速、高效地分离样品，并结合精确质量测量和串联质谱（MS/MS）。本文利用该技术对延胡索（一种重要的镇痛中药）中的生物碱进行鉴定。详细研究了一种叔胺生物碱和两种季铵生物碱的质谱裂解行为。对叔胺生物碱和季铵生物碱的低丰度产物离子进行了研究并相互比较。通过使用本实验室开发的系统筛选方法筛选出16种生物碱；其中8种生物碱的结构通过特征紫外吸收光谱和Q-TOF-MS/MS的正离子模式进行了鉴定；据我们所知，其中两种生物碱是首次在延胡索中发现的。本研究证明了UPLC-Q-TOF-MS在中药成分结构表征和鉴定方面的潜力。
