Yang Xia, Yuan Ruo, Chai Yaqin, Zhuo Ying, Hong Chenglin, Liu Zhongyuan, Su Huilan
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Talanta. 2009 Apr 30;78(2):596-601. doi: 10.1016/j.talanta.2008.12.017. Epub 2008 Dec 13.
A novel Cu(2)O-SiO(2) nanostructured particle was synthesized by a solution-phase method and was adopted for construction of a label-free amperometric immunosensor. The porous Cu(2)O-SiO(2) nanoparticles had good redox electrochemical activity, large surface-to-volume ratio, film-forming ability and high stability. The physical morphology and structure of Cu(2)O-SiO(2) nanoparticles were examined by scanning electron microscope (SEM) and transmission electron microscopy (TEM). The chemical component of Cu(2)O-SiO(2) was confirmed by X-ray photoelectron spectroscopy (XPS) and auger electron spectra (AES). The electrode modification process was probed by cyclic voltammetry (CV) and the performance of the immunosensor was studied by differential pulse voltammetry (DPV) measurements. To improve the analytical characteristics of the immunosensor, the experimental conditions were optimized. The immunosensor exhibited a good response to ferritin in ranges from 1.0 to 5.0 and 5.0 to 120.0 ng mL(-1) with a detection limit of 0.4 ng mL(-1). The fabricated immunosensor could make a low-cost, sensitive, quantitative detection of ferritin, and would have a potential application in clinical immunoassays.
通过溶液相法合成了一种新型的Cu(2)O-SiO(2)纳米结构粒子，并将其用于构建无标记安培免疫传感器。多孔的Cu(2)O-SiO(2)纳米粒子具有良好的氧化还原电化学活性、大的比表面积、成膜能力和高稳定性。通过扫描电子显微镜(SEM)和透射电子显微镜(TEM)对Cu(2)O-SiO(2)纳米粒子的物理形态和结构进行了检测。通过X射线光电子能谱(XPS)和俄歇电子能谱(AES)确定了Cu(2)O-SiO(2)的化学成分。通过循环伏安法(CV)探究电极修饰过程，并通过差分脉冲伏安法(DPV)测量研究免疫传感器的性能。为了改善免疫传感器的分析特性，对实验条件进行了优化。该免疫传感器对铁蛋白在1.0至5.0和5.0至120.0 ng mL(-1)范围内表现出良好的响应，检测限为0.4 ng mL(-1)。所制备的免疫传感器能够对铁蛋白进行低成本、灵敏、定量的检测，在临床免疫分析中具有潜在的应用价值。
