Qin Shunyi, Huang Kehe, Gao Jianzhong, Huang Da, Cai Tanxi, Pan Cuiling
Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China.
J Trace Elem Med Biol. 2009;23(1):29-35. doi: 10.1016/j.jtemb.2008.11.001. Epub 2009 Jan 23.
The experiment was conducted to compare the effect of different selenium sources on the expression of glutathione peroxidase 1 (GPx1) and iodothyronine deiodinase 1 (Dio1) mRNA in mice by quantitative real-time PCR. A total of 60 male Kunming mice at average body weight of 20 g were allotted to three groups in a randomized complete block design, namely two treatments and one control. Mice in Group 1 were fed a basal diet as control, while mice in Groups 2 and 3 were fed the basal diet supplemented with 0.1mg/kg selenium as sodium selenite or selenized yeast, respectively. Whole feeding experiment lasted for 30 d. At the end of the feeding trial, liver mRNA levels of GPx1 and Dio1 were determined by quantitative real-time PCR, as well as growth performance, body composition, blood and GPx activity were determined. The results showed that no significant differences in overall growth performance and body composition, including body weight, body length, heart weight, kidney weight and liver weight, were found between the experimental groups (P>0.05). Blood GPx activity increased in all of the selenium supplemented groups compared with control group (P<0.01). However, blood GPx activity in selenized yeast group was higher than that in sodium selenite group (P<0.05). Liver mRNA levels of GPx1 and Dio1 also increased in the two selenium supplemented groups compared with the control group (P<0.05), while there was no significant difference between the sodium selenite and selenized yeast groups (P>0.05). In conclusion, selenium increased the mRNA expression of GPx1 and Dio1 genes in murine liver, and there was no significant difference between the organic or inorganic form of selenium used.
本实验旨在通过定量实时聚合酶链反应（qRT-PCR）比较不同硒源对小鼠谷胱甘肽过氧化物酶1（GPx1）和碘甲状腺原氨酸脱碘酶1（Dio1）mRNA表达的影响。将60只平均体重为20 g的雄性昆明小鼠按照随机完全区组设计分为三组，即两种处理组和一个对照组。第1组小鼠喂食基础日粮作为对照，而第2组和第3组小鼠分别喂食添加0.1mg/kg亚硒酸钠或硒化酵母的基础日粮。整个饲养实验持续30天。在饲养试验结束时，通过定量实时聚合酶链反应测定肝脏中GPx1和Dio1的mRNA水平，并测定生长性能、身体组成、血液和GPx活性。结果表明，实验组之间在总体生长性能和身体组成方面，包括体重、体长、心脏重量、肾脏重量和肝脏重量，均未发现显著差异（P>0.05）。与对照组相比，所有添加硒的组血液中GPx活性均升高（P<0.01）。然而，硒化酵母组的血液GPx活性高于亚硒酸钠组（P<0.05）。与对照组相比，两个添加硒的组肝脏中GPx1和Dio1的mRNA水平也升高（P<0.05），而亚硒酸钠组和硒化酵母组之间没有显著差异（P>0.05）。总之，硒增加了小鼠肝脏中GPx1和Dio1基因的mRNA表达，并且所使用的有机或无机形式的硒之间没有显著差异。
