Mineral Nutrition Research Division, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, People's Republic of China.
Biol Trace Elem Res. 2012 Dec;150(1-3):158-65. doi: 10.1007/s12011-012-9517-3. Epub 2012 Oct 11.
An experiment is conducted to investigate the effects of selenium (Se) source and level on growth performance, tissue Se concentrations, antioxidation, and immune functions of heat-stressed broilers from 22 to 42 days of age. A total of 210 22-day-old Arbor Acres commercial male chicks were assigned by body weight to one of seven treatments with six replicates of five birds each in a completely randomized design involving a 3 × 2 factorial arrangement plus one Se-unsupplemented basal diet control (containing 0.027 mg of Se/kg). The three Se sources were sodium selenite (Na₂SeO₃), Se yeast, and AMMS Se (Se protein), and the two supplemental Se levels were 0.15 or 0.30 mg Se/kg. All birds were reared under heat-stressed condition (33 ± 1 °C during 0900-1700 hours and 27 ± 1 °C during 1900-0700 hours with a relative humidity of 60-80 %). The results showed that heat-stressed chicks fed Se-supplemented diets had higher (P < 0.10) average daily feed intake, Se concentrations in liver and breast muscle, liver glutathione peroxidase (GSH-Px) activity, serum antibody titers against H5N1(Re-4 strain), H5N1(Re-5 strain) and lower (P < 0.01) mortality compared with the control. Chicks fed the diets supplemented with 0.30 mg/kg of Se had higher (P < 0.05) Se concentrations in liver and breast muscle, liver GSH-Px activity, and serum antibody titer against H5N1 (Re-4 strain) than those fed the diets supplemented with 0.15 mg/kg of Se. Broilers fed the diets supplemented with Se yeast had higher (P < 0.001) Se concentrations in liver and breast muscle than those fed the diets supplemented with Na₂SeO₃ or AMMS Se. However, broilers fed the diets supplemented with AMMS Se had higher (P < 0.05) serum antibody titers against H5N1 (Re-4 strain) and H5N1 (Re-5 strain) than those fed the diets supplemented with Na₂SeO₃. These results indicated that Se yeast was more effective than Na₂SeO₃ or AMMS Se in increasing tissue Se retention; however, AMMS Se was more effective than Na₂SeO₃ or Se yeast in improving immune functions of heat-stressed broilers.
进行了一项实验，以研究硒（Se）源和水平对 22 至 42 日龄热应激肉鸡生长性能、组织 Se 浓度、抗氧化和免疫功能的影响。总共 210 只 22 日龄的 Arbor Acres 商业雄性小鸡按体重分配到 7 种处理中的一种，每个处理有 6 个重复，每个重复有 5 只鸡，采用完全随机设计，涉及 3×2 因子排列，外加一种不含硒的基础日粮对照（含 0.027 毫克/千克硒）。三种硒源分别为亚硒酸钠（Na₂SeO₃）、酵母硒和 AMMS Se（硒蛋白），两种补充硒水平分别为 0.15 或 0.30 毫克/千克硒。所有鸡均在热应激条件下饲养（0900-1700 小时期间 33±1°C，1900-0700 小时期间 27±1°C，相对湿度为 60-80%）。结果表明，饲喂添加硒的日粮的热应激小鸡的平均日采食量（P<0.10）、肝脏和胸肌中的硒浓度、肝脏谷胱甘肽过氧化物酶（GSH-Px）活性、血清对 H5N1（Re-4 株）、H5N1（Re-5 株）的抗体滴度均较高，死亡率较低（P<0.01）与对照组相比。饲喂 0.30 毫克/千克硒日粮的鸡的肝脏和胸肌中的硒浓度、肝脏 GSH-Px 活性和血清对 H5N1（Re-4 株）的抗体滴度均高于饲喂 0.15 毫克/千克硒日粮的鸡（P<0.05）。饲喂酵母硒日粮的鸡的肝脏和胸肌中的硒浓度均高于饲喂亚硒酸钠或 AMMS Se 日粮的鸡（P<0.001）。然而，饲喂 AMMS Se 日粮的鸡的血清对 H5N1（Re-4 株）和 H5N1（Re-5 株）的抗体滴度均高于饲喂亚硒酸钠日粮的鸡（P<0.05）。这些结果表明，酵母硒比亚硒酸钠或 AMMS Se 更有效地增加组织 Se 保留；然而，AMMS Se 比亚硒酸钠或酵母硒更有效地提高热应激肉鸡的免疫功能。
