Feed Science Institute, College of Animal Science, Zhejiang University, No. 388, Yuhangtang Road, Hangzhou 310058, People's Republic of China.
Poult Sci. 2012 Apr;91(4):936-42. doi: 10.3382/ps.2011-01921.
In total, 180 Lingnan Yellow broiler breeders were used to investigate the effect of different sources of selenium (Se) on the expression of cellular glutathione peroxidase (GPx1) and cytoplasmic thioredoxin reductase (TrxR1) in the liver and kidney of broiler breeders and their offspring by quantitative real-time PCR. There were 6 replicates of 3 dietary treatments. Broiler breeders were fed corn-soy-based diets supplemented with 0.15 mg/kg of Se from sodium selenite, Se-enriched yeast (SY), or selenomethionine (SM). At the end of the feeding trial, liver and kidney mRNA levels of GPx1 and TrxR1 were determined by quantitative real-time PCR, as well as the activity of GPx1 and TrxR1 in liver and kidney of breeders and their offspring. The results showed that, compared with sodium selenite, SY or SM significantly increased (P < 0.05) the activity of TrxR1 in the liver and kidney of broiler breeders and their offspring but not the GPx1 activity. The liver GPx1 and TrxR1 mRNA levels in SY or SM groups were higher (P < 0.05) than that in the sodium selenite group. And the kidney TrxR1 mRNA levels were also significantly increased (P < 0.05) by using SY or SM, whereas there was no significant difference in the kidney GPx1 mRNA levels between the organic or inorganic sources of Se used.
总共使用了 180 只岭南黄肉鸡种鸡来研究不同硒源（Se）对种鸡及其后代肝脏和肾脏中细胞谷胱甘肽过氧化物酶（GPx1）和细胞质硫氧还蛋白还原酶（TrxR1）表达的影响，采用定量实时 PCR 法进行分析。有 6 个日粮处理组，每个处理组有 3 个重复。种鸡饲喂补充有 0.15 mg/kg 硒的玉米-豆粕基础日粮，硒源分别为亚硒酸钠、富硒酵母（SY）或硒代蛋氨酸（SM）。在饲养试验结束时，通过定量实时 PCR 测定种鸡及其后代肝脏和肾脏中 GPx1 和 TrxR1 的 mRNA 水平，以及种鸡及其后代肝脏和肾脏中 GPx1 和 TrxR1 的活性。结果表明，与亚硒酸钠相比，SY 或 SM 显著提高（P < 0.05）了种鸡及其后代肝脏和肾脏中 TrxR1 的活性，但对 GPx1 活性没有影响。SY 或 SM 组的肝脏 GPx1 和 TrxR1 mRNA 水平均高于亚硒酸钠组（P < 0.05），而 SY 或 SM 组的肾脏 TrxR1 mRNA 水平也显著升高（P < 0.05），但两种有机或无机硒源对肾脏 GPx1 mRNA 水平的影响没有显著差异。
