Fujino Yasuhito, Liao Chun-Peng, Zhao Yan Shi, Pan Judong, Mathes Lawrence E, Hayes Kathleen A, Ohno Koichi, Tsujimoto Hajime, Roy-Burman Pradip
Department of Pathology, University of Southern California School of Medicine, Los Angeles, CA 90033, USA.
Virology. 2009 Mar 30;386(1):16-22. doi: 10.1016/j.virol.2009.01.021. Epub 2009 Feb 8.
A new proviral integration site for feline leukemia virus (FeLV), termed flit-1, was identified from feline thymic lymphoma. Among 35 FeLV-related tumors examined, 5 of 25 thymic lymphomas demonstrated proviral insertion within flit-1 locus whereas none of four alimentary and five multicentric lymphomas and one T-lymphoid leukemia examined had rearrangement in this region. Extensive sequence analysis has shown that flit-1, which is noncoding, is conserved on human chromosome 12 and mouse chromosome 15. The human and murine homologs of flit-1 are positioned approximately 30-kb upstream to activin-A receptor type II-like 1 (ACVRL1/ALK1) gene. Expression of ACVRL1 mRNA was examined in two of five lymphomas with flit-1 rearrangement and detected in both of the two whereas normal thymuses and seven lymphoid tumors without flit-1 rearrangement had no detectable expression. Therefore, flit-1 appears to represent a novel FeLV proviral common integration domain that may influence lymphomagenesis as insertional mutagenesis.
从猫胸腺淋巴瘤中鉴定出一种新的猫白血病病毒（FeLV）前病毒整合位点，称为flit-1。在检测的35个与FeLV相关的肿瘤中，25个胸腺淋巴瘤中有5个显示前病毒插入flit-1基因座，而在检测的4个消化道淋巴瘤、5个多中心淋巴瘤和1个T淋巴细胞白血病中，该区域均未发生重排。广泛的序列分析表明，非编码的flit-1在人类12号染色体和小鼠15号染色体上保守。flit-1的人类和小鼠同源物位于激活素A受体II型样1（ACVRL1/ALK1）基因上游约30 kb处。在5个有flit-1重排的淋巴瘤中的2个中检测了ACVRL1 mRNA的表达，在这2个中均检测到表达，而正常胸腺和7个无flit-1重排的淋巴瘤未检测到表达。因此，flit-1似乎代表了一种新的FeLV前病毒共同整合域，可能作为插入诱变影响淋巴瘤的发生。
