Huang Xiao-Ming, Dai Cheng-Bo, Mou Zhong-Lin, Wang Li-Juan, Wen Wei-Ping, Lin Shu-Guang, Xu Geng, Li Hua-Bin
Allergy and Cancer Center, Otorhinolaryngology Hospital of The First Affiliated Hospital of Sun Yat-sen University and Otorhinolaryngology Institute of Sun Yat-sen University, Guangzhou 510080, China.
Cancer Lett. 2009 Jun 28;279(1):47-56. doi: 10.1016/j.canlet.2009.01.020. Epub 2009 Feb 8.
Activated mTOR was implicated to play a role in the carcinogenesis of nasopharyngeal carcinoma (NPC). However, the mechanism of activated mTOR/Complex1(mTORC1) signaling pathway in NPC development has not been well established. In this study, we correlated the expression of mTORC1 signal molecules and Cyclin D1 in NPC. We also investigated the effect of blocking mTORC1 signal with rapamycin and mTOR siRNA on Cyclin D1 expression in CNE-2 cells, as well as cell apoptosis and viability. We found a positive association of mTORC1 signal molecules and Cyclin D1 in NPC. Also, we found blockage mTORC1 inhibited Cyclin D1 expression in CNE-2 cells and enhanced cell apoptosis. Our results suggested that mTORC1 signal pathway might be a potential target for NPC therapy.
激活的mTOR被认为在鼻咽癌（NPC）的致癌过程中发挥作用。然而，激活的mTOR/复合物1（mTORC1）信号通路在NPC发展中的机制尚未完全明确。在本研究中，我们关联了NPC中mTORC1信号分子与细胞周期蛋白D1的表达。我们还研究了用雷帕霉素和mTOR小干扰RNA阻断mTORC1信号对CNE-2细胞中细胞周期蛋白D1表达以及细胞凋亡和活力的影响。我们发现NPC中mTORC1信号分子与细胞周期蛋白D1呈正相关。此外，我们发现阻断mTORC1可抑制CNE-2细胞中细胞周期蛋白D1的表达并增强细胞凋亡。我们的结果表明，mTORC1信号通路可能是NPC治疗的一个潜在靶点。
