Bio-Nano Electronics Research Center, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama 350-8585, Japan.
J Biomater Appl. 2010 Feb;24(6):527-44. doi: 10.1177/0885328208101340. Epub 2009 Feb 9.
Poly (ethylene terephthalate) (PET) was surface modified by plasma polymerization of acetobromo-alpha-D-glucose (ABG) at different radio frequency (RF) powers. Plasma polymerization was carried out by vaporizing ABG in the powder form by heating at 135 degrees C. Surface modification resulted in improved hydrophilicity and smoothness of the surface especially at low RF powers (30-50 W), but at high RF powers, the surface was found to be etched and the hydrophilicity decreased as evidenced by atomic force microscopy (AFM) and contact angle measurements. The plasma polymerized ABG film was found to be extensively cross-linked as evidenced by its insolubility in water. Infra red (IR) and X-ray photoelectron spectroscopy (XPS) were employed to characterize the plasma polymerized ABG films. IR studies revealed that at lower RF powers, polymerization was taking place mainly by breaking up of acetoxy group while retaining the ring structures to a major extent during the polymerization process whereas at high RF powers, the rupture of ring structures was indicated. XPS indicated a reduction in the percentage of oxygen in the polymers going from low to high RF powers suggestive of complete destruction of the acetoxy group at high RF powers. Cross-cut tests showed excellent adhesive properties of the plasma polymerized ABG films onto PET. Static platelet adhesion tests using platelet rich human plasma showed significantly reduced adhesion of platelets onto modified PET surface as evidenced by scanning electron microscopy. Polymerization of glucose and its derivatives using RF plasma has not been reported so far and the preliminary results reported in this study shows that this could be an interesting approach in the surface modification of biomaterials.
聚对苯二甲酸乙二醇酯（PET）通过在不同射频（RF）功率下对乙酰溴-α-D-葡萄糖（ABG）进行等离子体聚合来进行表面改性。等离子体聚合通过在 135°C 加热时将 ABG 以粉末形式蒸发来进行。表面改性导致表面的亲水性和光滑度得到改善，尤其是在低 RF 功率（30-50W）下，但在高 RF 功率下，表面被发现被蚀刻，亲水性降低，如原子力显微镜（AFM）和接触角测量所示。等离子体聚合的 ABG 膜被发现是广泛交联的，这一点可以通过其在水中不溶来证明。红外（IR）和 X 射线光电子能谱（XPS）用于表征等离子体聚合的 ABG 膜。IR 研究表明，在较低的 RF 功率下，聚合主要通过打破乙酰氧基基团发生，而在聚合过程中主要保留环结构，而在较高的 RF 功率下，表明环结构的破裂。XPS 表明，随着 RF 功率从低到高，聚合物中氧的百分比减少，这表明在高 RF 功率下完全破坏了乙酰氧基基团。十字切割测试显示等离子体聚合的 ABG 膜对 PET 具有极好的粘附性能。使用富含血小板的人血浆进行的静态血小板粘附测试表明，血小板在改性 PET 表面上的粘附显著减少，这可以通过扫描电子显微镜证明。到目前为止，还没有报道使用 RF 等离子体聚合葡萄糖及其衍生物的情况，本研究中的初步结果表明，这可能是生物材料表面改性的一种有趣方法。
