Verdaguer N, Aymami J, Fernández-Forner D, Fita I, Coll M, Huynh-Dinh T, Igolen J, Subirana J A
Departament d'Enginyeria Química, Universitat Politècnica de Catalunya, Barcelona, Spain.
J Mol Biol. 1991 Sep 20;221(2):623-35. doi: 10.1016/0022-2836(91)80077-8.
We have determined the crystal structure of the dodecamer d(CCCCCGCGGGGG), showing for the first time a complete turn of A-DNA. It has average structural parameters similar to those determined in fibres. Nevertheless it shows a considerable local variation in structure which is in part associated with the presence of a bound spermine molecule. We conclude that the local DNA conformation does not only depend on the base sequence, but may be strongly modified upon interaction with other molecules. In particular, the CpG sequence, which is found in hypersensitive regions of the genome, appears to be able to easily change its conformation under external influences.
我们已经确定了十二聚体d(CCCCCGCGGGGG)的晶体结构，首次展示了完整一圈的A-DNA。它具有与纤维中测定的平均结构参数相似的参数。然而，它在结构上显示出相当大的局部变化，部分与结合的精胺分子的存在有关。我们得出结论，局部DNA构象不仅取决于碱基序列，而且在与其他分子相互作用时可能会受到强烈修饰。特别是，在基因组超敏区域发现的CpG序列似乎能够在外部影响下轻易改变其构象。
