Kalchayanand Norasak, Arthur Terrance M, Bosilevac Joseph M, Brichta-Harhay Dayna M, Guerini Michael N, Shackelford Steven D, Wheeler Tommy L, Koohmaraie Mohammad
U.S. Department of Agriculture, Agricultural Research Service, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, Nebraska 68933-0166, USA.
J Food Prot. 2009 Jan;72(1):151-6. doi: 10.4315/0362-028x-72.1.151.
1,3-Dibromo-5,5-dimethylhydantoin (DBDMH; 25 degrees C) and hot water (85 degrees C) spray treatments were evaluated for efficacy in decontamination of pathogenic bacteria attached to beef carcass surfaces represented by cutaneous trunci (CT) muscle sections and beef hearts. Treatments were evaluated using two different systems, a commercial carcass wash cabinet and a model carcass washer. The effects were measured immediately after treatment and again after 48 h of storage at 4 degrees C. Section of CT and beef hearts were inoculated with bovine fecal solution containing approximately 6 log CFU/cm2 of Escherichia coli O157:H7 and Salmonella. After DBDMH or hot water spray treatments, bacterial populations were enumerated immediately and after storage for 48 h at 4 degrees C. DBDMH treatments reduced aerobic plate counts, Enterobacteriaceae, E. coli O157: H7, and Salmonella by the same or slightly lower amounts relative to hot water treatment. DBDMH reduced aerobic plate counts and Enterobacteriaceae by 2.8 to 3.6 log CFU/cm2, E. coli O157:H7 by 1.6 to 2.1 log CFU/cm2, and Salmonella by 0.7 to 2.3 log CFU/cm2 on CT sections and beef hearts. Hot water treatment reduced aerobic plate counts and Enterobacteriaceae by 3.0 to 4.1 log CFU/cm2, E. coli O157:H7 by 1.8 to 2.3 log CFU/cm2, and Salmonella by 2.5 to 2.8 log CFU/cm2. After 48 h of storage, the reductions of organisms by DBDMH and hot water treatments were not different. This study demonstrated that DBDMH spray washing could be effective as an antimicrobial intervention for beef carcasses and variety meats.
评估了1,3 - 二溴 - 5,5 - 二甲基乙内酰脲（DBDMH；25摄氏度）和热水（85摄氏度）喷雾处理对附着在以躯干皮肤（CT）肌肉切片和牛心为代表的牛肉胴体表面的病原菌的去污效果。使用两种不同的系统进行处理评估，一种是商用胴体清洗柜，另一种是模拟胴体清洗机。在处理后立即以及在4摄氏度储存48小时后再次测量效果。将CT切片和牛心接种含有约6 log CFU/cm²大肠杆菌O157:H7和沙门氏菌的牛粪溶液。在DBDMH或热水喷雾处理后，立即和在4摄氏度储存48小时后对细菌数量进行计数。相对于热水处理，DBDMH处理使需氧平板计数、肠杆菌科、大肠杆菌O157:H7和沙门氏菌减少的量相同或略低。DBDMH使CT切片和牛心上的需氧平板计数和肠杆菌科减少2.8至3.6 log CFU/cm²，大肠杆菌O157:H7减少1.6至2.1 log CFU/cm²，沙门氏菌减少0.7至2.3 log CFU/cm²。热水处理使需氧平板计数和肠杆菌科减少3.0至4.1 log CFU/cm²，大肠杆菌O157:H7减少1.8至2.3 log CFU/cm²，沙门氏菌减少2.5至2.8 log CFU/cm²。储存48小时后，DBDMH和热水处理对微生物的减少量没有差异。这项研究表明，DBDMH喷雾清洗作为牛肉胴体和牛杂碎的抗菌干预措施可能是有效的。
