Carlson Brandon A, Ruby John, Smith Gary C, Sofos John N, Bellinger Gina R, Warren-Serna Wendy, Centrella Bill, Bowling Rod A, Belk Keith E
Center for Meat Safety & Quality, Department of Animal Sciences, Colorado State University, Fort Collins, Colorado 80523-1171, USA.
J Food Prot. 2008 Nov;71(11):2223-7. doi: 10.4315/0362-028x-71.11.2223.
This study involved a comparison of the antimicrobial efficacy of several beef hide decontamination interventions to identify those that more effectively reduced levels of Escherichia coli O157:H7 and Salmonella. Whole beef hides were inoculated with E. coli O157:H7 and Salmonella and decontaminated with sprays of solutions of acetic acid (AA; 10%, 55 degrees C), lactic acid (LA; 10%, 55 degrees C), sodium hydroxide (SH; 3%, 23 degrees C), sodium metasilicate (SM; 4%, 23 degrees C), or sodium hydroxide (1.5%), followed by high-pressure washing with chlorinated (0.02%) water (SHC; both applied at 23 degrees C) or water (W; 23 degrees C) or by deluging with solutions of potassium cyanate (PC; 2.4%, 30 degrees C) or sodium sulfide (SS; 6.2%, 30 degrees C). All spraying treatments (AA, LA, SH, SM, and SHC) resulted in removal of visual organic material, whereas the dehairing treatments (PC and SS) successfully removed hair along with visual organic material. The PC, SS, and SHC treatments resulted in the greatest reductions of E. coli O157:H7 (P < 0.05), by 5.1, 4.8, and 5.0 log CFU/cm2, respectively. The SS and SHC treatments decreased Salmonella by 4.2 and 4.4 log CFU/cm2, respectively, compared with the water treatment, which reduced levels by 1.7 log CFU/cm2 (P < 0.05). The SH, AA, and LA treatments also lowered both E. coli O157:H7 and Salmonella by at least 2.0 log CFU/cm2. The treatments that were effective in this study deserve further consideration for commercial implementation as hide decontamination interventions.
本研究对几种牛皮去污干预措施的抗菌效果进行了比较，以确定那些能更有效地降低大肠杆菌O157:H7和沙门氏菌水平的措施。将完整的牛皮接种大肠杆菌O157:H7和沙门氏菌，并用醋酸（AA；10%，55℃）、乳酸（LA；10%，55℃）、氢氧化钠（SH；3%，23℃）、偏硅酸钠（SM；4%，23℃）或氢氧化钠（1.5%）溶液喷雾进行去污，然后用含氯（0.02%）水（SHC；均在23℃下应用）或水（W；23℃）进行高压冲洗，或用氰酸钾（PC；2.4%，30℃）或硫化钠（SS；6.2%，30℃）溶液浸泡。所有喷雾处理（AA、LA、SH、SM和SHC）都能去除可见的有机物质，而去毛处理（PC和SS）则成功去除了毛发以及可见的有机物质。PC、SS和SHC处理使大肠杆菌O157:H7的减少量最大（P<0.05），分别减少了5.1、4.8和5.0 log CFU/cm2。与水处理相比，SS和SHC处理分别使沙门氏菌减少了4.2和4.4 log CFU/cm2，水处理使沙门氏菌水平降低了1.7 log CFU/cm2（P<0.05）。SH、AA和LA处理也使大肠杆菌O157:H7和沙门氏菌的数量至少降低了2.0 log CFU/cm2。本研究中有效的处理措施值得作为牛皮去污干预措施进一步考虑用于商业应用。
