Mitsui Nobuo, Murasawa Hisashi, Sekiguchi Junichi
Department of Bioscience and Textile Technology, Interdisciplinary Graduate School of Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda-shi, Nagano 386-8567, Japan.
Appl Microbiol Biotechnol. 2009 Mar;82(4):741-8. doi: 10.1007/s00253-009-1894-y. Epub 2009 Feb 10.
The effects of cortex-lysis related genes with the pdaA, sleB, and cwlD mutations of Bacillus subtilis (natto) NAFM5 on sporulation and germination were investigated. Single or double mutations did not prevent normal sporulation, but did affect germination. Germination was severely inhibited by the double mutation of sleB and cwlD. The quality of natto made with the sleB cwlD double mutant was tested, and the amounts of glutamic acid and ammonia were very similar to those in the wild type. The possibility of industrial development of natto containing a reduced number of viable spores is presented.
研究了纳豆芽孢杆菌NAFM5的pdaA、sleB和cwlD突变的皮层溶解相关基因对芽孢形成和萌发的影响。单突变或双突变并不妨碍正常的芽孢形成，但会影响萌发。sleB和cwlD的双突变严重抑制了萌发。对用sleB cwlD双突变体制成的纳豆质量进行了测试，谷氨酸和氨的含量与野生型非常相似。提出了含有减少数量活孢子的纳豆进行工业开发的可能性。
