Hosoi T, Ametani A, Kiuchi K, Kaminogawa S
Tokyo Metropolitan Food Technology Research Center, Japan.
Can J Microbiol. 1999 Jan;45(1):59-66.
We examined changes in mouse fecal microflora after various dietary components and Bacillus subtilis (natto) spores were delivered by intubation. The administration of intact spores of Bacillus subtilis (natto) did not affect fecal Enterobacteriaceae and Enterococcus spp. in all three diet groups; on the other hand, it did affect fecal Bacteroidaceae and Lactobacillus spp., depending upon the diets fed. The administration of autoclaved spores did not alter fecal microflora. In vitro cultures of Lactobacillus murinus obtained from mouse feces, together with Bacillus subtilis (natto) under aerobic conditions as a mixed culture, revealed that the growth of L. murinus was enhanced by the addition of intact spores of Bacillus subtilis (natto). This enhancement of growth was displayed only in media containing either sucrose, glucose, maltose, or fructose but not in media containing cornstarch, soluble starch, or microcrystalline cellulose. From these results it was evident that some metabolites of Bacillus subtilis (natto) produced during germination and (or) outgrowth of spores of this strain, requiring monosaccharides or oligosaccharides, participated in the enhancement of the growth of L. murinus.
我们通过插管给予小鼠不同饮食成分和枯草芽孢杆菌（纳豆芽孢杆菌）孢子后，检测了小鼠粪便微生物群的变化。在所有三个饮食组中，给予完整的枯草芽孢杆菌（纳豆芽孢杆菌）孢子均未影响粪便中的肠杆菌科和肠球菌属；另一方面，根据所喂食的饮食不同，它确实影响了粪便中的拟杆菌科和乳酸杆菌属。给予经高压灭菌的孢子未改变粪便微生物群。从小鼠粪便中获得的鼠李糖乳杆菌与枯草芽孢杆菌（纳豆芽孢杆菌）在有氧条件下作为混合培养物进行体外培养，结果显示，添加完整的枯草芽孢杆菌（纳豆芽孢杆菌）孢子可促进鼠李糖乳杆菌的生长。这种生长促进仅在含有蔗糖、葡萄糖、麦芽糖或果糖的培养基中出现，而在含有玉米淀粉、可溶性淀粉或微晶纤维素的培养基中未出现。从这些结果可以明显看出，枯草芽孢杆菌（纳豆芽孢杆菌）在孢子萌发和（或）生长过程中产生的一些代谢产物，需要单糖或寡糖参与，从而促进了鼠李糖乳杆菌的生长。
