Hopfgartner Gérard, Varesio Emmanuel, Stoeckli Markus
Life Sciences Mass Spectrometry, School of Pharmaceutical Sciences, University of Geneva, Geneva, Switzerland.
Rapid Commun Mass Spectrom. 2009 Mar;23(6):733-6. doi: 10.1002/rcm.3934.
The fast imaging of complete rat sections by matrix-assisted laser desorption/ionization on a triple quadrupole linear ion trap is demonstrated. After administration of the pharmaceutical compound (MW=467.4 u) at 0.5 mg/kg the parent drug could be identified in full scan mode and in the enhanced product ion spectrum mode. Furthermore, the precursor ion mode could also be used to monitor the presence of the parent drug in the tissue section. In the selected reaction monitoring mode, using a laser frequency of 1000 Hz and a rastering speed of about 18 mm/s, a targeted representative image of drug distribution in a rat section could be obtained in less than 15 min.
展示了在三重四极杆线性离子阱上通过基质辅助激光解吸/电离对完整大鼠切片进行快速成像。以0.5 mg/kg的剂量给予药物化合物（分子量=467.4 u）后，可在全扫描模式和增强产物离子谱模式下鉴定出母体药物。此外，前体离子模式也可用于监测组织切片中母体药物的存在。在选择反应监测模式下，使用1000 Hz的激光频率和约18 mm/s的光栅扫描速度，可在不到15分钟的时间内获得大鼠切片中药物分布的靶向代表性图像。
