Dilmetz Brooke A, Lee Yea-Rin, Condina Mark R, Briggs Matthew, Young Clifford, Desire Christopher T, Klingler-Hoffmann Manuela, Hoffmann Peter
Future Industries Institute University of South Australia Adelaide Australia.
Clinical and Health Sciences, Health and Biomedical Innovation University of South Australia Adelaide Australia.
Anal Sci Adv. 2021 Mar 16;2(3-4):225-237. doi: 10.1002/ansa.202000176. eCollection 2021 Apr.
The applicability of mass spectrometry imaging (MSI) has exponentially increased with the improvement of sample preparation, instrumentation (spatial resolution) and data analysis. The number of MSI publications listed in PubMed continues to grow with 378 published articles in 2020-2021. Initially, MSI was just sensitive enough to identify molecular features correlating with distinct tissue regions, similar to the resolution achieved by visual inspection after standard immunohistochemical staining. Although the spatial resolution was limited compared with other imaging modalities, the molecular intensity mapping added a new exciting capability. Over the past decade, significant improvements in every step of the workflow and most importantly in instrumentation were made, which now enables the molecular analysis at a cellular and even subcellular level. Here, we summarize the latest developments in MSI, with a focus on the latest approaches for tissue-based imaging described in 2020.
随着样品制备、仪器设备（空间分辨率）和数据分析的改进，质谱成像（MSI）的适用性呈指数级增长。PubMed上列出的MSI出版物数量持续增加，2020年至2021年有378篇发表的文章。最初，MSI的灵敏度仅足以识别与不同组织区域相关的分子特征，类似于标准免疫组织化学染色后目视检查所达到的分辨率。尽管与其他成像方式相比，空间分辨率有限，但分子强度映射增加了一项令人兴奋的新功能。在过去十年中，工作流程的每一步都有了显著改进，最重要的是仪器设备方面，现在能够在细胞甚至亚细胞水平上进行分子分析。在这里，我们总结了MSI的最新进展，重点关注2020年描述的基于组织成像的最新方法。
