Wilson Robert, Spiller David G, Prior Ian A, Veltkamp Kees J, Hutchinson Alistair
Department of Chemistry, Liverpool University, Liverpool L69 7ZD, UK.
ACS Nano. 2007 Dec;1(5):487-93. doi: 10.1021/nn700289m.
This report describes a simple method for preparing encoded microspheres for use in multiplexed biological detection. In this method, hydrophobic trioctylphosphine oxide (TOPO)-capped CdSe@ZnS quantum dots (QDs) are assembled on polyamine-coated microspheres in chloroform and encapsulated in an outer shell of silica nanoparticles functionalized with a specific recognition surface. Because TOPO-capped QDs are assembled instead of their water-soluble equivalents, the microspheres are highly luminescent. The amount of QDs assembled depends only on the surface area of the substrate, and therefore, the photoluminescence intensity increased uniformly in proportion to the number of QD layers assembled. The outer shell of silica nanoparticles confers stability on the assembled QDs but has no effect on their photoluminescence because it is transparent to excitatory and emitted light. It was activated with aminosilane and functionalized with a recognition surface of protein antigens using disulfide exchange chemistry. Magnetic beads furnished with spectral codes of up to three colors of QDs matched to specific recognition surfaces were used as multianalyte sensors for serum proteins.
本报告描述了一种制备用于多重生物检测的编码微球的简单方法。在该方法中，疏水性三辛基氧化膦（TOPO）包覆的CdSe@ZnS量子点（QDs）在氯仿中组装在聚胺包覆的微球上，并封装在具有特定识别表面功能化的二氧化硅纳米颗粒外壳中。由于组装的是TOPO包覆的量子点而非其水溶性等效物，微球具有高发光性。组装的量子点数量仅取决于底物的表面积，因此，光致发光强度与组装的量子点层数成正比均匀增加。二氧化硅纳米颗粒外壳赋予组装的量子点稳定性，但由于其对激发光和发射光透明，对其光致发光没有影响。它用氨基硅烷活化，并使用二硫键交换化学方法用蛋白质抗原的识别表面进行功能化。配备有与特定识别表面匹配的多达三种颜色量子点光谱代码的磁珠用作血清蛋白的多分析物传感器。
