Bao Jie, Chen Wei, Liu Taotao, Zhu Yulin, Jin Peiyuan, Wang Leyu, Liu Junfeng, Wei Yongge, Li Yadong
Department of Chemistry, Tsinghua University, Beijing, P. R. China.
ACS Nano. 2007 Nov;1(4):293-8. doi: 10.1021/nn700189h.
In this article, we report the synthesis of bifunctional Au-Fe(3)O(4) nanoparticles that are formed by chemical bond linkage. Due to the introduction of Au nanoparticles, the resulting bifunctional Au-Fe(3)O(4) nanoparticles can be easily modified with other functional molecules to realize various nanobiotechnological separations and detections. Here, as an example, we demonstrate that as-prepared Au-Fe(3)O(4) nanoparticles can be modified with nitrilotriacetic acid molecules through Au-S interaction and used to separate proteins simply with the assistance of a magnet. Bradford protein assay and sodium dodecyl sulfate-polyacrylamide gel electrophoresis were performed to examine the validity of the separation procedure, and the phosphate determination method suggested that the as-separated protein maintained catalytic activity. This result shows the efficiency of such a material in protein separation and suggests that its use can be extended to magnetic separation of other biosubstances. Moreover, this synthetic strategy paves the way for facile preparation of diverse bifunctional and even multifunctional nanomaterials.
在本文中，我们报道了通过化学键连接形成的双功能金-四氧化三铁纳米颗粒的合成。由于金纳米颗粒的引入，所得的双功能金-四氧化三铁纳米颗粒可以很容易地用其他功能分子进行修饰，以实现各种纳米生物技术的分离和检测。在此，作为一个例子，我们证明了所制备的金-四氧化三铁纳米颗粒可以通过金-硫相互作用用次氮基三乙酸分子进行修饰，并在磁铁的辅助下简单地用于分离蛋白质。进行了考马斯亮蓝蛋白测定和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳以检验分离程序的有效性，并且磷酸盐测定方法表明所分离的蛋白质保持催化活性。该结果显示了这种材料在蛋白质分离中的效率，并表明其用途可以扩展到其他生物物质的磁分离。此外，这种合成策略为简便制备各种双功能甚至多功能纳米材料铺平了道路。
