Yagami Akiko, Inaba Yasuko, Kuno Yuki, Suzuki Kayoko, Tanaka Akira, Sjolander Sigrid, Saito Hirohisa, Matsunaga Kayoko
Department of Dermatology, Fujita Health University School of Medicine, Aichi, Japan.
J Dermatol. 2009 Jan;36(1):50-5. doi: 10.1111/j.1346-8138.2008.00585.x.
Oral allergy syndrome to soy milk is classified as a phenotype of pollen-food allergy syndrome (PFAS). As causative antigens, Gly m 4 (Bet v 1 homolog, 17 kD) and oleosin (23 kD), have been reported. In this study, we report two cases of PFAS to soy milk. Both cases showed positive reactions to soy milk in skin prick tests (SPT) and to Gly m 4 in specific serum immunoglobulin (Ig)E antibody. When we measured specific serum IgE antibody of soy-related proteins using a new laboratory testing method, microarray analysis, both cases showed a positive reaction for Bet v 1. One case was weakly positive for a soybean protein, beta-conglycinin. Other results for reactivity to soy, peanut, cross-reactive carbohydrate determinants and profilin were negative. Based on these results, we diagnosed the two cases as PFAS to Gly m 4. We also performed protein microarray analysis and found it useful as a screening test for immediate allergy, such as PFAS.
豆奶口腔过敏综合征被归类为花粉 - 食物过敏综合征（PFAS）的一种表型。作为致病抗原，已报道了大豆球蛋白Gly m 4（与Bet v 1同源，17 kD）和油质蛋白（23 kD）。在本研究中，我们报告了2例对豆奶的PFAS病例。两例患者在皮肤点刺试验（SPT）中对豆奶以及在特异性血清免疫球蛋白（Ig）E抗体检测中对Gly m 4均呈阳性反应。当我们使用一种新的实验室检测方法——微阵列分析来检测大豆相关蛋白的特异性血清IgE抗体时，两例患者对Bet v 1均呈阳性反应。其中1例对大豆蛋白β-伴大豆球蛋白呈弱阳性。对大豆、花生、交叉反应性碳水化合物决定簇和肌动蛋白结合蛋白的其他反应结果均为阴性。基于这些结果，我们将这两例患者诊断为对Gly m 4的PFAS。我们还进行了蛋白质微阵列分析，发现它作为一种对速发型过敏（如PFAS）的筛查试验很有用。
