Ha Seon-Ah, Lee Youn Soo, Shin Seung Min, Kim Hyun Kee, Kim Sanghee, Namkoong Hong, Kim Hae Joo, Jung Sang Min, Lee Yu Sun, Chung Yeun Jun, Jung Sang Seol, Kim Jin Woo
Department of Molecular Genetic Laboratory, College of Medicine, The Catholic University of Korea, Seoul, Korea.
BMC Cancer. 2009 Feb 11;9:51. doi: 10.1186/1471-2407-9-51.
Oncoprotein HCCR-1 functions as a negative regulator of the p53 and contributes breast tumorigenesis. The serum HCCR-1 assay is useful in diagnosing breast cancer and mice transgenic for HCCR developed breast cancers. But it is unknown how HCCR-1 contributes to human breast tumorigenesis.
Oncogene HCCR-1 expression levels were determined in normal breast tissues, breast cancer tissues and cancer cell lines. We examined whether HCCR-1 protein expression in breast cancer is related to different biological characteristics, including ER, PR, p53 genotype, and HER2 status in 104 primary breast cancer tissues using immunohistochemical analyses.
HCCR-1 was upregulated in breast cancer cells and tissues compared with normal breast tissues. In this study, overexpression of HCCR-1 was well correlated with known breast cancer prognostic markers including the presence of steroid receptors (ER and PR), p53 mutation and high HER2 overexpression. HCCR-1 was not detected in the ER-negative, PR-negative, p53 negative and low HER2 breast cancer tissues. These data indicate that the level of HCCR-1 in breast cancer tissues is relatively well correlated with known breast cancer factors, including the HER2 overexpression, p53 mutation, and ER/PR status.
Determination of HCCR-1 levels as options for HER2 testing is promising although it needs further evaluation.
癌蛋白HCCR - 1作为p53的负调节因子发挥作用，并促进乳腺癌的发生。血清HCCR - 1检测在乳腺癌诊断中具有重要价值，且携带HCCR基因的转基因小鼠会发生乳腺癌。但目前尚不清楚HCCR - 1如何促进人类乳腺癌的发生。
测定正常乳腺组织、乳腺癌组织及癌细胞系中癌基因HCCR - 1的表达水平。我们采用免疫组织化学分析方法，检测了104例原发性乳腺癌组织中HCCR - 1蛋白表达是否与不同生物学特征相关，包括雌激素受体（ER）、孕激素受体（PR）、p53基因型及人表皮生长因子受体2（HER2）状态。
与正常乳腺组织相比，乳腺癌细胞和组织中HCCR - 1表达上调。在本研究中，HCCR - 1的过表达与已知的乳腺癌预后标志物密切相关，包括类固醇受体（ER和PR）的存在、p53突变及HER2高表达。在ER阴性、PR阴性、p53阴性及HER2低表达的乳腺癌组织中未检测到HCCR - 1。这些数据表明，乳腺癌组织中HCCR - 1水平与已知的乳腺癌相关因素，包括HER2过表达、p53突变及ER/PR状态相对密切相关。
尽管需要进一步评估，但将HCCR - 1水平测定作为HER2检测的一种选择具有一定前景。
