Llanos Carolina, Chan Edward K L, Li Songqing, Abadal Grant X, Izmirly Peter, Byrne Caroline, Clancy Robert M, Buyon Jill P
Department of Medicine, New York University School of Medicine, New York, NY 10016, USA.
J Rheumatol. 2009 Mar;36(3):565-9. doi: 10.3899/jrheum.080860. Epub 2009 Feb 4.
To evaluate the frequency of anti-alpha-enolase antibodies in the sera of mothers whose children have congenital heart block (CHB), given provocative results in which alpha-enolase, a membrane protein, was recognized by monoclonal antibodies reactive with the peptide p200 of 52 kDa Ro/SSA in a neonatal rat heart library.
An ELISA using a recombinant alpha-enolase protein was developed. Sera from 100 anti-Ro52+ CHB mothers in the Research Registry for Neonatal Lupus, 50 patients with systemic lupus erythematosus (SLE; 7 anti-Ro52+), and 48 healthy controls were tested for anti-alpha-enolase reactivity.
There were no significant differences in the median values obtained from CHB mothers, patients with SLE, or controls at each of the dilutions tested. Only 7 (7%) at 1:100 dilution and 2 (2%) at 1:1000 dilution of 100 CHB sera were 3 standard deviations above the mean value obtained for controls. Preincubation with recombinant Ro52 did not inhibit anti-alpha-enolase reactivity.
The low frequency of anti-alpha-enolase antibodies in the sera of CHB mothers and the absence of apparent cross-reactivity with Ro52 suggest that antibodies to Ro52 are not likely to mediate CHB via binding to alpha-enolase.
鉴于在新生大鼠心脏文库中，一种膜蛋白α-烯醇化酶可被与52 kDa Ro/SSA的p200肽反应的单克隆抗体识别，这一结果令人关注，本研究旨在评估患有先天性心脏传导阻滞（CHB）患儿的母亲血清中抗α-烯醇化酶抗体的频率。
开发了一种使用重组α-烯醇化酶蛋白的酶联免疫吸附测定（ELISA）。对新生儿狼疮研究登记处的100名抗Ro52阳性的CHB母亲、50名系统性红斑狼疮（SLE）患者（7名抗Ro52阳性）和48名健康对照者的血清进行抗α-烯醇化酶反应性检测。
在每个测试稀释度下，CHB母亲、SLE患者或对照者获得的中位数无显著差异。100份CHB血清中，仅1:100稀释度下有7份（7%）、1:1000稀释度下有2份（2%）高于对照均值3个标准差。与重组Ro52预孵育并未抑制抗α-烯醇化酶反应性。
CHB母亲血清中抗α-烯醇化酶抗体频率较低，且与Ro52无明显交叉反应，这表明抗Ro52抗体不太可能通过与α-烯醇化酶结合介导CHB。
