先天性心脏传导阻滞中母体和新生儿抗Ro(SSA)及La(SSB)抗体的亚类分布

Subclass distribution of maternal and neonatal anti-Ro(SSA) and La(SSB) antibodies in congenital heart block.

作者信息

Tseng C E, Caldwell K, Feit S, Chan E K, Buyon J P

机构信息

Department of Medicine, New York University Medical Center, New York, USA.

出版信息

J Rheumatol. 1996 May;23(5):925-32.

PMID:8724310

Abstract

OBJECTIVE

To compare the subclass distribution of anti-48 kDa La(SSB) and anti-52 and 60 kDa Ro(SSA) antibodies in the maternal and neonatal circulation, in pregnancies affected and unaffected by the development of congenital heart block (CHB).

METHODS

Sera were obtained from 32 mothers (during 34 pregnancies 23 complicated by CHB and 11 healthy) demonstrated to have anti-Ro(SSA) and/or La(SSB). Maternal and neonatal autoantibodies were evaluated for subclass distribution by ELISA:

RESULTS

All 4 subclasses of anti-Ro(SSA) and La(SSB) antibodies cross the placenta and are detectable in sera obtained from the umbilical cord, IgG1 and IgG3 were the major subclasses represented in the 48 kDa La(SSB) and 52 kDa Ro(SSA) responses. All subclasses, including IgG2 and IgG4, were observed in about one-third of the anti-52 kDa Ro(SSA) and 48 kDa La(SSB) responses. In contrast, anti-60 kDa antibodies were, with rare exception, confined to IgG1. Except for anti-48 kDa La(SSB) IgG3 antibodies, no significant differences were observed between affected and unaffected pregnancies in the ratio of maternal to neonatal levels of any of the antibody subclasses. Overall, there were no significant differences in the subclass profiles between mothers whose children had heart block and those who did not.

CONCLUSION

The IgG subclasses of anti-48 kDa La(SSB) and anti-52 and 60 kDa Ro(SSA) do not account for the susceptibility of one fetus versus another for the development of CHB. Anti-60 kDa Ro(SSA) antibodies are more restricted in subclass distribution than anti-52 kDA Ro(SSA) or 48 kDa La(SSB) responses.

摘要

目的

比较先天性心脏传导阻滞（CHB）发生与否的妊娠中，母体和新生儿循环中抗48 kDa La（SSB）及抗52和60 kDa Ro（SSA）抗体的亚类分布。

方法

从32名母亲（34次妊娠，其中23次合并CHB，11次为健康妊娠）中获取血清，这些母亲均被证实有抗Ro（SSA）和/或La（SSB）。通过酶联免疫吸附测定（ELISA）评估母体和新生儿自身抗体的亚类分布。

结果

抗Ro（SSA）和La（SSB）抗体的所有4个亚类均可穿过胎盘，并可在脐带血清中检测到。IgG1和IgG3是48 kDa La（SSB）和52 kDa Ro（SSA）反应中的主要亚类。在约三分之一的抗52 kDa Ro（SSA）和48 kDa La（SSB）反应中观察到了包括IgG2和IgG4在内的所有亚类。相比之下，抗60 kDa抗体除极少数例外，仅限于IgG1。除抗48 kDa La（SSB）IgG3抗体外，在受影响和未受影响的妊娠中，任何抗体亚类的母体与新生儿水平之比均未观察到显著差异。总体而言，孩子有心脏传导阻滞的母亲与孩子没有心脏传导阻滞的母亲之间，亚类谱没有显著差异。