Diamanti-Kandarakis Evanthia, Piouka Athanasia, Livadas Sarantis, Piperi Christine, Katsikis Ilias, Papavassiliou Athanasios G, Panidis Demetrios
Endocrine Section, First Department of Medicine, University of Athens Medical School, Mikras Asias 75, Goudi 115-27, Athens, Greece.
Eur J Endocrinol. 2009 May;160(5):847-53. doi: 10.1530/EJE-08-0510. Epub 2009 Feb 10.
Oocyte maturation process characterizes polycystic ovary syndrome (PCOS). The mechanisms of this abnormality leading to chronic anovulation are under investigation. Advanced glycosylated end products (AGEs), a marker of oxidative stress linked with oocyte maturation are localized in granulosa cells and are increased in sera, in women with PCOS. The aim of this study was to investigate the relationship, whether there is an association between the anti-mullerian hormone (AMH), a hormone produced by granulosa cells and AGEs in ovulatory and anovulatory PCOS (PCOS-Anov), as well as in non-PCOS anovulatory (Non-PCOS Anov) women. Design Cross-sectional study.
Data from sixty women with PCOS (37 anovulatory and 23 regularly ovulating) were compared with eleven Non-PCOS Anov women and 25 normal women. In each subject biochemical, hormonal, and ultrasonographic parameters were studied.
AMH values were statistically significantly higher in PCOS-Anov (7.63+/-3.12) in comparison with ovulatory PCOS (PCOS-Ov; 4.92+/-2.50), Non-PCOS Anov (3.66+/-1.4), and controls (4.02+/-1.27 ng/ml). AGEs demonstrated a similar pattern: 8.70+/-1.65 in PCOS-Anov, 7.43+/-1.79, PCOS-Ov, 5.21+/-0.09, Non-PCOS Anov, and 5.85+/-0.89 U/ml in controls (P<0.005 for all comparison respectively). Follicle number was significantly higher in PCOS-Anov in comparison with other groups. A significant positive correlation between AMH and AGEs was observed (r: 0.326, P<0.01), and with the estimated AMH/AGEs ratio to follicle number (r: 0.42, P: 0.0001) and the presence of anovulation.
These data suggest that an oxidative marker, AGEs, and AMH, may interact in the anovulatory mechanisms in women with PCOS.
卵母细胞成熟过程是多囊卵巢综合征（PCOS）的特征。导致慢性无排卵的这种异常机制正在研究中。晚期糖基化终产物（AGEs）是一种与卵母细胞成熟相关的氧化应激标志物，定位于颗粒细胞中，且在PCOS女性的血清中含量升高。本研究的目的是调查颗粒细胞产生的抗苗勒管激素（AMH）与排卵型和无排卵型PCOS（PCOS - Anov）以及非PCOS无排卵（Non - PCOS Anov）女性中AGEs之间的关系，是否存在关联。设计横断面研究。
将60例PCOS女性（37例无排卵和23例规律排卵）的数据与11例非PCOS无排卵女性和25例正常女性的数据进行比较。研究了每个受试者的生化、激素和超声参数。
与排卵型PCOS（PCOS - Ov；4.92±2.50）、非PCOS无排卵（3.66±1.4）和对照组（4.02±1.27 ng/ml）相比，PCOS - Anov组的AMH值在统计学上显著更高（7.63±3.12）。AGEs呈现类似模式：PCOS - Anov组为8.70±1.65，PCOS - Ov组为7.43±1.79，非PCOS无排卵组为5.21±0.09，对照组为5.85±0.89 U/ml（所有比较P<0.005）。PCOS - Anov组的卵泡数量显著高于其他组。观察到AMH与AGEs之间存在显著正相关（r：0.326，P<0.01），且与估计的AMH/AGEs比值与卵泡数量（r：0.42，P：0.0001）以及无排卵的存在相关。
这些数据表明，氧化应激标志物AGEs和AMH可能在PCOS女性的无排卵机制中相互作用。
