Hyde G K, McCullen S D, Jeon S, Stewart S M, Jeon H, Loboa E G, Parsons G N
Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, USA.
Biomed Mater. 2009 Apr;4(2):025001. doi: 10.1088/1748-6041/4/2/025001. Epub 2009 Feb 11.
Atomic layer deposition (ALD) is investigated as a process to produce inorganic metallic bio-adhesive coatings on cellulosic fiber substrates. The atomic layer deposition technique is known to be capable of forming highly conformal and uniform inorganic thin film coatings on a variety of complex surfaces, and this work presents an initial investigation of ALD on porous substrate materials to produce high-precision biocompatible titanium oxynitride coatings. X-ray photoelectron spectroscopy (XPS) confirmed TiNOx composition, and transmission electron microscopy (TEM) analysis showed the coatings to be uniform and conformal on the fiber surfaces. Biocompatibility of the modified structures was determined as a function of coating layer thickness by fluorescent live/dead staining of human adipose-derived adult stem cells (hADSC) at 6, 12 and 24 h. Cell adhesion showed that thin TiNOx coatings yielded the highest number of cells after 24 h with a sample coated with a 20 A coating having approximately 28.4 +/- 3.50 ng DNA. By altering the thickness of the deposited film, it was possible to control the amount of cells adhered to the samples. This work demonstrates the potential of low temperature ALD as a surface modification technique to produce biocompatible cellulose and other implant materials.
研究了原子层沉积（ALD）作为一种在纤维素纤维基材上制备无机金属生物粘附涂层的工艺。众所周知，原子层沉积技术能够在各种复杂表面上形成高度保形且均匀的无机薄膜涂层，并且这项工作对在多孔基材材料上进行ALD以制备高精度生物相容性氮氧化钛涂层进行了初步研究。X射线光电子能谱（XPS）证实了TiNOx的组成，透射电子显微镜（TEM）分析表明涂层在纤维表面上是均匀且保形的。通过对人脂肪来源的成体干细胞（hADSC）在6、12和24小时进行荧光活/死染色，确定了改性结构的生物相容性与涂层厚度的关系。细胞粘附实验表明，在24小时后，薄的TiNOx涂层产生的细胞数量最多，涂有20埃涂层的样品含有约28.4±3.50纳克DNA。通过改变沉积膜的厚度，可以控制粘附在样品上的细胞数量。这项工作证明了低温ALD作为一种表面改性技术在生产生物相容性纤维素和其他植入材料方面的潜力。
