Faverani Leonardo P, Astaneh Sarah Hashemi, Costa Monique Gonçalves da, Delanora Leonardo A, Lima-Neto Tiburtino J, Barbosa Stéfany, Ariani Maretaningtias Dwi, Takoudis Christos, Sukotjo Cortino
Department of Diagnosis and Surgery, School of Dentistry, Sao Paulo State University (UNESP), Aracatuba 16015-050, Brazil.
Department of Chemical Engineering, University of Illinois Chicago, Chicago, IL 60612, USA.
J Funct Biomater. 2023 Feb 23;14(3):120. doi: 10.3390/jfb14030120.
The membranes used in bone reconstructions have been the object of investigation in the field of tissue engineering, seeking to improve their mechanical strength and add other properties, mainly the osteopromotive. This study aimed to evaluate the functionalization of collagen membranes, with atomic layer deposition of TiO on the bone repair of critical defects in rat calvaria and subcutaneous biocompatibility. A total of 39 male rats were randomized into four groups: blood clot (BC), collagen membrane (COL), COL 150-150 cycles of titania, and COL 600-600 cycles of titania. The defects were created in each calvaria (5 mm in diameter) and covered according to each group; the animals were euthanized at 7, 14, and 28 days. The collected samples were assessed by histometric (newly bone formed, soft tissue area, membrane area, and residual linear defect) and histologic (inflammatory cells and blood cells count) analysis. All data were subjected to statistical analysis ( < 0.05). The COL150 group showed statistically significant differences compared to the other groups, mainly in the analysis of residual linear defects (1.5 ± 0.5 × 10 pixels/µm for COL 150, and around 1 ± 0.5 × 10 pixels/µm for the other groups) and newly formed bone (1500 ± 1200 pixels/µm for COL 150, and around 4000 pixels/µm for the others) ( < 0.05), demonstrating a better biological behavior in the chronology of defects repair. It is concluded that the collagen membrane functionalized by TiO over 150 cycles showed better bioactive potential in treating critical size defects in the rats' calvaria.
用于骨重建的膜一直是组织工程领域的研究对象，旨在提高其机械强度并添加其他特性，主要是骨诱导特性。本研究旨在评估胶原膜的功能化，通过在大鼠颅骨关键缺损的骨修复和皮下生物相容性方面进行二氧化钛原子层沉积。总共39只雄性大鼠被随机分为四组：血凝块（BC）、胶原膜（COL）、二氧化钛150 - 150循环的COL组以及二氧化钛600 - 600循环的COL组。在每只颅骨上制造缺损（直径5毫米）并根据每组情况进行覆盖；在第7、14和28天对动物实施安乐死。通过组织计量学（新形成的骨、软组织面积、膜面积和残余线性缺损）和组织学（炎症细胞和血细胞计数）分析对收集的样本进行评估。所有数据均进行统计分析（<0.05）。与其他组相比，COL150组显示出统计学上的显著差异，主要体现在残余线性缺损分析（COL 150为1.5±0.5×10像素/微米，其他组约为1±0.5×10像素/微米）和新形成的骨（COL 150为1500±1200像素/微米，其他组约为4000像素/微米）方面（<0.05），表明在缺损修复进程中具有更好的生物学行为。结论是，经150次以上循环二氧化钛功能化的胶原膜在治疗大鼠颅骨临界尺寸缺损方面显示出更好的生物活性潜力。
