Lu Wenjing, Wang Hongtao, Huang Changyong, Reichardt W
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China.
J Environ Sci (China). 2008;20(12):1487-93. doi: 10.1016/s1001-0742(08)62554-1.
Forty-six candidate phenol/benzoate degrading-iron reducing bacteria were isolated from long-term irrigated tropical paddy soils by enrichment procedures. Pure cultures and some prepared mixed cultures were examined for ferric oxide reduction and phenol/benzoate degradation. All the isolates were iron reducers, but only 56.5% could couple iron reduction to phenol and/or benzoate degradation, as evidenced by depletion of phenol and benzoate after one week incubation. Analysis of degradative capability using Biolog MT plates revealed that most of them could degrade other aromatic compounds such as ferulic acid, vanillic acid, and hydroxybenzoate. Mixed-cultures and soil samples displayed greater capacity for aromatic degradation and iron reduction than pure bacterial isolates, suggesting that these reactions may be coupled via a consortia-based mechanism in paddy soils.
通过富集培养程序，从长期灌溉的热带稻田土壤中分离出46株候选苯酚/苯甲酸盐降解-铁还原细菌。对纯培养物和一些制备好的混合培养物进行了氧化铁还原和苯酚/苯甲酸盐降解检测。所有分离菌株均能还原铁，但只有56.5%的菌株能将铁还原与苯酚和/或苯甲酸盐降解偶联，这在一周培养后苯酚和苯甲酸盐的消耗中得到了证明。使用Biolog MT平板分析降解能力表明，它们中的大多数能够降解其他芳香族化合物，如阿魏酸、香草酸和羟基苯甲酸盐。混合培养物和土壤样品比纯细菌分离物表现出更大的芳香族降解和铁还原能力，这表明在稻田土壤中这些反应可能通过基于菌群的机制偶联。
