El-Assal Faiza M, El-Lakwah Soheir F, Hasheesh Wafaa S, El-Mahdi Magda
Department of Zoology, Faculty of Science, Cairo University, Egypt.
J Egypt Soc Parasitol. 2008 Dec;38(3):929-44.
The free-living infective juveniles of the entomopathogenic nematodes are non-feeding, so the stored energy reserves in these juveniles are of great importance. The relationship between the energy reserves and the efficacy of the entomopathogenic nematodes of genera Heterorhabditis and Steinernema was studied. New progenies of S. riobrave and H. bacteriophora (ISK-2 strains), were obtained using the continuous culturing method of nematode juveniles (IJs), for the several cycles under the optimum condition of the temperature (25 degrees C) and nematode density (20 IJs/ larva), inside the host Galleria mellonella. Thus, the nematode efficacy was maximized with a high conservation of energy reserves. The results showed that there was an increase in the penetration rate and the virulence through the new progenies of S. riobrave and H. bacteriophora than in the original progenies, with an increase in the energy reserves.
昆虫病原线虫的自由生活感染性幼虫不进食，因此这些幼虫体内储存的能量储备非常重要。研究了能量储备与异小杆线虫属和斯氏线虫属昆虫病原线虫效力之间的关系。在寄主大蜡螟体内温度（25摄氏度）和线虫密度（20条感染性幼虫/幼虫）的最佳条件下，采用线虫幼虫（感染性幼虫）连续培养方法，经过几个周期，获得了斯氏短体线虫和嗜菌异小杆线虫（ISK - 2菌株）的新后代。因此，线虫效力在能量储备高度保守的情况下达到最大化。结果表明，与原始后代相比，斯氏短体线虫和嗜菌异小杆线虫的新后代的穿透率和毒力随着能量储备的增加而提高。
