Mejia-Torres M C, Sáenz A
Laboratorio de Control Biológico, Pontificia Universidad Javeriana - HPUJ, Cra 7a No 43-82 Ed 54 (Jesús Emilio Ramírez), Colombia.
Braz J Biol. 2013 May;73(2):239-43. doi: 10.1590/S1519-69842013000200003.
The entomopathogenic nematode Heterorhabditis sp. SL0708 (Rhabditida: Heterorhabditidae) isolated from soil in Alcalá, Valle del Cauca (Colombia) was characterised ecologically using Galleria mellonella larvae (L) (Pyralidae: Galleriinae) as hosts. The effect of temperature on the viability, infectivity and reproduction, and of moisture on infectivity and storage in liquid were evaluated in infective juveniles (IJs). Significant differences were found in the viability, infectivity and reproduction of the IJs at different temperatures. No nematodes were recovered at 5 °C and 10 °C, and at 35 °C no infectivity was observed. Average daily nematode recovery was best at 25 °C, and survival of the IJs was low in substrates presenting 13% moisture. The optimal storage temperature for Heterorhabditis sp. SL0708 was between 20 °C and 30 °C, keeping its infectivity for up to 8 weeks.
从哥伦比亚考卡山谷省阿尔卡拉的土壤中分离出的昆虫病原线虫斯氏异小杆线虫（Heterorhabditis sp.）SL0708（小杆目：异小杆线虫科），以大蜡螟幼虫（L）（螟蛾科：蜡螟亚科）为宿主进行了生态特征研究。在感染性幼虫（IJ）中评估了温度对其活力、感染性和繁殖的影响，以及湿度对感染性和液体保存的影响。在不同温度下，IJ的活力、感染性和繁殖存在显著差异。在5℃和10℃时未回收线虫，在35℃时未观察到感染性。25℃时平均每日线虫回收率最佳，在湿度为13%的基质中IJ的存活率较低。斯氏异小杆线虫SL0708的最佳保存温度在20℃至30℃之间，其感染性可保持长达8周。
