Department of Chemistry, University of Oxford, Chemistry Research Laboratory, UK.
J Am Chem Soc. 2009 Mar 4;131(8):2758-9. doi: 10.1021/ja8061369.
Amphiphilic donor-acceptor meso-ethynyl porphyrins with polar pyridinium electron-acceptor head groups and hydrophobic dialkyl-aniline electron donors have high molecular hyperpolarizabilities (as measured by hyper-Rayleigh scattering) and high affinities for biological membranes. When bound to water droplets in dodecane, or to the plasma membranes of living cells, they can be used for second harmonic generation (SHG) microscopy; an incident light of wavelength 840 nm generates a strong frequency-doubled signal at 420 nm. Copper(II) and nickel(II) porphyrin complexes give similar SHG signals to those of the free-base porphyrins, while exhibiting no detectable two-photon excited fluorescence.
具有亲水头基（带极性吡啶鎓电子受体基团）和疏水底基（二烷基苯胺电子给体）的给体-受体型meso-乙基取代卟啉，具有高的分子超极化率（通过超瑞利散射测量），对生物膜具有高亲和力。当它们与十二烷中的水滴结合，或与活细胞的质膜结合时，可用于二次谐波产生（SHG）显微镜；840nm 波长的入射光在 420nm 处产生强的倍频信号。铜（II）和镍（II）卟啉配合物产生与游离卟啉相似的 SHG 信号，而没有检测到双光子激发荧光。
