Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Oxford, UK.
Biomed J. 2022 Oct;45(5):749-762. doi: 10.1016/j.bj.2022.05.007. Epub 2022 Jun 3.
The brain is the most unexplored part of our body. The lack of sufficient tools has hindered our understanding of the brain and the associated diseases. The study of neurons and the neuronal network will help elucidate how the brain functions and related disorders. Over the last few decades, an increasing number of techniques have been reported to study neurons and neuronal communication in vitro, ex vivo, and in vivo. These methods have pushed the boundaries of neuroscience and elucidated more information than ever before; however, much more requires to be done to understand the brain in its entirety. In this review article, I discuss the principles and the advantages and disadvantages of the classical electrode-based recording techniques and the optical imaging-based methods, which have aided neuroscientists in understanding neuronal communication.
大脑是我们身体中最未被探索的部分。缺乏足够的工具阻碍了我们对大脑和相关疾病的理解。对神经元和神经元网络的研究将有助于阐明大脑的功能和相关障碍。在过去的几十年中，已经有越来越多的技术被报道用于在体外、离体和体内研究神经元和神经元通讯。这些方法推动了神经科学的发展，揭示了比以往更多的信息；然而，要全面了解大脑，还需要做更多的工作。在这篇综述文章中，我讨论了基于经典电极的记录技术和基于光学成像的方法的原理以及它们的优缺点，这些方法帮助神经科学家理解神经元通讯。
