Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, 4200 Hwy 97 Summerland, BC, Canada V0H 1Z0.
J Agric Food Chem. 2009 Mar 11;57(5):1805-13. doi: 10.1021/jf803467y.
A bench-scale pressurized low polarity water (PLPW) extractor was used for the extraction and separation of hemicellulose, cellulose, lignin, and other phenolic compounds in flax shives. In the first part of this research, the key PLPW extraction process variables of temperature, pH, and flow rate, were optimized using central composite design (CCD). Temperature and pH of water had a significant affect on the fractionation of carbohydrates (cellulose and hemicellulose), lignin, and other phenolics. The optimal extraction conditions for the separation of hemicellulose and lignin, determined by the optimization using CCD, were 170 degrees C, pH 3.0, and a flow rate of 2.5 mL/min. Under these extraction conditions, 39.3% of the initial biomass or feed, 70.1% of the hemicellulose, 35.3% of the lignin, and 5.3% of the cellulose were extracted from the flax shives. In order to improve the purity and yield of the cellulose, a two-stage PLPW extraction was examined. The first stage was designed to remove hemicellulose by water at 170 degrees C and the second stage was intended for delignification by a pH 12 buffer at 220 degrees C. The two-stage PLPW extraction effectively removed 63.2% of the feed, 97.3% of hemicellulose, and 86.3% of lignin, while solubilizing 23.9% of cellulose; resulting in a solid residue containing 0.7 g of hemicellulose, 3.5 g of lignin, and 27.3 g of cellulose/100 g of DFS. The PLPW extraction is able to extract and separate components in flax shives by changing pH and temperature. The best case occurs between pH 9.5 and 12, resulting in maximum solubilization of hemicellulose and lignin.
采用中试规模加压低极性水（PLPW）萃取器从亚麻屑中提取和分离半纤维素、纤维素、木质素和其他酚类化合物。在本研究的第一部分，使用中心复合设计（CCD）优化了温度、pH 值和流速等关键 PLPW 萃取工艺变量。水的温度和 pH 值对半纤维素（纤维素和半纤维素）、木质素和其他酚类化合物的分级有显著影响。通过 CCD 优化确定的用于分离半纤维素和木质素的最佳萃取条件为 170°C、pH3.0 和流速 2.5mL/min。在这些萃取条件下，39.3%的初始生物质或进料、70.1%的半纤维素、35.3%的木质素和 5.3%的纤维素从亚麻屑中被提取出来。为了提高纤维素的纯度和得率，考察了两级 PLPW 萃取。第一级设计用于在 170°C 下用水去除半纤维素，第二级设计用于在 220°C 下用 pH12 缓冲液进行脱木质素。两级 PLPW 萃取有效地去除了 63.2%的进料、97.3%的半纤维素和 86.3%的木质素，同时溶解了 23.9%的纤维素；得到的固体残渣含有 0.7g 半纤维素、3.5g 木质素和 27.3g 纤维素/100g DFS。PLPW 萃取可以通过改变 pH 值和温度来提取和分离亚麻屑中的成分。最佳情况出现在 pH9.5 到 12 之间，导致半纤维素和木质素的最大溶解。
