Department of Internal Medicine, Divisions of Endocrinology and Metabolism, Division of Biomathematics, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA.
J Neuroendocrinol. 1989 Jun 1;1(3):185-94. doi: 10.1111/j.1365-2826.1989.tb00101.x.
Abstract We have examined the co-pulsatility of luteinizing hormone (LH) and prolactin, LH and follicle-stimulating hormone (FSH), and LH and alpha subunit in normal men. We tested whether the degree of physiologically observed co-pulsatility (peak coincidence) significantly exceeded expected random concordance between independently pulsating hormone series. To this end, computer simulations were used to create synthetic endocrine time series pulsating randomly and independently at known frequencies. Resultant predictions of the mean, variance and probability distribution of the number of randomly coincident peaks permitted us to test the null hypothesis that physiologically observed hormone co-pulsatility was due to chance peak associations alone. Physiological observations were made in 33 normal men and in six ovariectomized ewes subjected to combined hypothalamo-pituitary and jugular venous catheterization. The following salient results were obtained: 1) random peak coincidence rates between independently pulsating hormone series were substantial at high pulse frequencies, but such random rates were significantly exceeded in the case of gonadotropin-releasing hormone and LH peaks (P< 0.0001); 2) random coincidence was further increased when coincidence was defined as peak maxima occurring not only simultaneously but also within some defined time window (e.g. +/-10 min, as commonly done in the literature); 3) significant co-pulsatility could be demonstrated for simultaneous LH and FSH pulsations in normal men (P< 0.0001); 4) coincidence rates for 10-min lagged (but not for simultaneous) LH and prolactin pulses were significantly more likely than chance associations; 5) observed coincidence between LH and a subunit pulses significantly exceeded expected (random) peak overlap (P<0.001); and 6) in contrast, hormone peaks in different men were only randomly associated. We conclude that based upon the means, variances and probability distributions calculated here, available reports on peak coincidence between pulsatile neuroendocrine time series must be re-examined in the light of high rates of random coincidence observed between independently pulsating hormone series.
摘要 我们研究了正常男性促黄体生成素（LH）和催乳素、LH 和卵泡刺激素（FSH）以及 LH 和α亚基之间的共脉冲性。我们检验了生理观察到的共脉冲性（峰重合）是否显著超过独立脉冲激素系列之间随机一致性的预期。为此，我们使用计算机模拟创建了以已知频率随机独立脉冲的合成内分泌时间序列。由此产生的关于随机重合峰数量的平均值、方差和概率分布的预测，使我们能够检验这样一个假设，即生理观察到的激素共脉冲性仅归因于随机峰关联。生理观察是在 33 名正常男性和 6 名接受下丘脑-垂体和颈静脉导管联合置管的去卵巢绵羊中进行的。得到以下主要结果：1）在高脉冲频率下，独立脉冲激素系列之间的随机峰重合率相当高，但在促性腺激素释放激素和 LH 峰的情况下，这种随机率显著增加（P<0.0001）；2）当重合定义为不仅同时而且在某些定义的时间窗口内（例如 +/-10 分钟，如文献中常见）出现的峰最大值时，随机重合进一步增加；3）在正常男性中可以证明 LH 和 FSH 同时脉冲的显著共脉冲性（P<0.0001）；4）LH 和催乳素 10 分钟滞后（而非同时）脉冲的重合率比随机关联更有可能；5）观察到的 LH 和α亚基脉冲之间的重合显著超过预期（随机）峰重叠（P<0.001）；6）相反，不同男性的激素峰仅随机相关。我们的结论是，根据这里计算的平均值、方差和概率分布，必须重新检查关于脉冲神经内分泌时间序列之间峰重合的现有报告，因为在独立脉冲激素系列之间观察到了很高的随机重合率。
