来自荧光团螺旋的偏振及其与从肌肉获得的偏振的关系。
Polarization from a helix of fluorophores and its relation to that obtained from muscle.
作者信息
Tregear R T, Mendelson R A
机构信息
University of California, San Francisco, California 94143, USA.
出版信息
Biophys J. 1975 May;15(5):455-67. doi: 10.1016/S0006-3495(75)85830-9. Epub 2009 Jan 1.
Abstract
Expressions for the polarization of fluorescence from fluorophores in a helical array have been obtained. These predictions have been compared with experimental data obtained by reacting fluorescent S-1 subfragments of myosin (extrinsically labeled with N-(iodoacetylaminoethyl)-5-napthylamine-1-sulfonic acid [1,5-IAEDANS]) with glycerated rabbit psoas fibers in rigor. A comparison with data obtained by directly labeling fibers with this fluorophore is also presented.
摘要
已获得螺旋阵列中荧光团荧光偏振的表达式。这些预测结果已与通过使肌球蛋白的荧光S-1亚片段（用N-（碘乙酰氨基乙基）-5-萘胺-1-磺酸[1,5-IAEDANS]进行外部标记）与处于僵直状态的甘油化兔腰大肌纤维反应而获得的实验数据进行了比较。还给出了与用这种荧光团直接标记纤维所获得的数据的比较。
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