Nareyeck Gordon, Zeschnigk Michael, Bornfeld Norbert, Anastassiou Gerasimos
Department of Ophthalmology, Universitätsklinikum Essen, Essen, Germany.
Ophthalmologica. 2009;223(3):196-201. doi: 10.1159/000201566. Epub 2009 Feb 12.
Uveal melanomas with or without monosomy 3 have different metastatic potentials. Therefore, it would be of great experimental importance to obtain cell lines of both groups - established and characterized under the same conditions.
Long-term culture of biopsies derived from untreated primary uveal melanomas was performed, and the status of chromosome 3 in the tumour was determined. Characterization by immunocytochemistry and in vitro assays for cell migration, vasculogenic cord formation and proliferation were performed.
Establishment of cell lines succeeded in 6 out of 128 uveal melanomas. No histopathological feature was predictive for cultivation success. Two out of 4 cell lines derived from tumours with monosomy 3 were invasive in vitro. Both cell lines with disomy 3 showed higher proliferation levels (p = 0.002).
The in vitro tendency for invasion did not correlate with the proliferative behaviour of uveal melanoma cell lines, but was partly associated with monosomy of chromosome 3.
伴有或不伴有3号染色体单体型的葡萄膜黑色素瘤具有不同的转移潜能。因此，在相同条件下建立并鉴定这两组细胞系具有重要的实验意义。
对未经治疗的原发性葡萄膜黑色素瘤活检组织进行长期培养，并确定肿瘤中3号染色体的状态。通过免疫细胞化学和体外细胞迁移、血管生成索形成及增殖分析进行鉴定。
128例葡萄膜黑色素瘤中有6例成功建立细胞系。没有组织病理学特征可预测培养成功。来自具有3号染色体单体型肿瘤的4个细胞系中有2个在体外具有侵袭性。两个具有3号染色体二体的细胞系显示出更高的增殖水平（p = 0.002）。
葡萄膜黑色素瘤细胞系的体外侵袭倾向与增殖行为无关，但部分与3号染色体单体型有关。
