Nareyeck Gordon, Zeschnigk Michael, Prescher Gabriele, Lohmann Dietmar R, Anastassiou Gerasimos
Department of Ophthalmology, University of Duisburg-Essen, Augenklinik, Universitaetsklinikum Essen, Hufelandstrasse 55, D-45122 Essen, Germany.
Exp Eye Res. 2006 Oct;83(4):858-64. doi: 10.1016/j.exer.2006.04.004. Epub 2006 Jun 5.
Uveal melanoma (UM) is the most common intraocular malignancy. Approximately 50% of UM patients die of metastases, which mainly arise from primary tumors with loss of an entire chromosome 3 (monosomy 3). To identify cell lines with monosomy 3 that may serve as a model system for UM with high metastatic potential, we determined the chromosome 3 status of previously established and frequently used UM cell lines by microsatellite analysis (Mel202, Mel285, Mel290, 92-1, OMM-1, OCM-1, OCM-3, OCM-8) and cytogenetic analysis (Mel202, Mel285, OCM-8). We found that none of these cell lines has monosomy 3. Therefore we established and characterized two novel cell lines, UPMM-1 and UPMM-2 that are both developed from primary uveal melanoma tissue samples with monosomy 3. The cell line UPMM-1 has retained the chromosome 3 status of the primary tumor. In UPMM-2 chromosome 3 has undergone duplication (isodisomy) and is present on the background of a hypotetraploid karyotype. Our data suggest that, UPMM-1 may serve as a model system to study the mechanisms underlying the metastatic potential of uveal melanomas with monosomy 3.
葡萄膜黑色素瘤（UM）是最常见的眼内恶性肿瘤。约50%的UM患者死于转移，转移主要源于整条3号染色体缺失（3号染色体单体性）的原发性肿瘤。为了鉴定具有3号染色体单体性且可作为高转移潜能UM模型系统的细胞系，我们通过微卫星分析（Mel202、Mel285、Mel290、92 - 1、OMM - 1、OCM - 1、OCM - 3、OCM - 8）和细胞遗传学分析（Mel202、Mel285、OCM - 8）确定了先前建立并经常使用的UM细胞系的3号染色体状态。我们发现这些细胞系均无3号染色体单体性。因此，我们建立并鉴定了两个新的细胞系，UPMM - 1和UPMM - 2，它们均由具有3号染色体单体性的原发性葡萄膜黑色素瘤组织样本培养而来。细胞系UPMM - 1保留了原发性肿瘤的3号染色体状态。在UPMM - 2中，3号染色体发生了重复（等二体性），并存在于亚四倍体核型的背景中。我们的数据表明，UPMM - 1可作为研究具有3号染色体单体性的葡萄膜黑色素瘤转移潜能潜在机制的模型系统。
