Liu She-Lan, Dong Ying, Zhang Li, Li Min-Wei, Wo Jian-er, Lu Li-Wei, Chen Zhen-Juan, Wang Yong-Zhong, Ruan Bing
Department of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, 310003, Hangzhou, People's Republic of China.
Arch Virol. 2009;154(3):437-43. doi: 10.1007/s00705-009-0315-y. Epub 2009 Feb 11.
The influence of hepatitis B virus (HBV) gene heterogeneity on the failure of HBV vaccination in eastern China remains unknown. Here, we assigned 78 hepatitis B surface antigen (HBsAg)-carrier mothers to two groups: 41 mothers from whom transmission of HBV to their children was successfully prevented and 37 mothers whose children were HBsAg positive 1 year after HBV vaccination. The DNA loads in mothers of the failure group (4.17E + 07 copies/ml) were significantly higher than those in the success group (8.40E + 06 copies/ml). However, no difference was found in the S gene mutation rate and genotypes between the groups. Interestingly, Thr123Ala and Gly145Arg were observed only in failure-group mothers, whereas Thr126Asn, Thr126Ser, Thr143Asn, Asp144Gly, and Asp144Ala were seen in the success group. Thus, high viral load is an important risk factor for HBV vaccination failure, which is correlated with the positions of mutations in the S gene, but not with mutant frequencies or genotypes.
乙肝病毒（HBV）基因异质性对中国东部地区乙肝疫苗接种失败的影响尚不清楚。在此，我们将78名乙肝表面抗原（HBsAg）携带者母亲分为两组：41名成功预防了HBV向其子女传播的母亲，以及37名其子女在接种乙肝疫苗1年后HBsAg呈阳性的母亲。失败组母亲的DNA载量（4.17E + 07拷贝/毫升）显著高于成功组（8.40E + 06拷贝/毫升）。然而，两组之间的S基因突变率和基因型未发现差异。有趣的是，仅在失败组母亲中观察到Thr123Ala和Gly145Arg，而在成功组中则发现了Thr126Asn、Thr126Ser、Thr143Asn、Asp144Gly和Asp144Ala。因此，高病毒载量是乙肝疫苗接种失败的一个重要危险因素，这与S基因中的突变位置相关，但与突变频率或基因型无关。
